The squalene content of pumpkin seed oil is affected by the method of extraction
and was found to be high in oils extracted from husk seeds than in de-hulled/naked
seeds (Nakić et al. 2006). Nawirska-Olszańska et al. (2013) reported that pumpkin
seeds contain cardiac glycosides, terpenoids, and resins. Pumpkin seeds also contain
glycosides viz. cucurbitacin L 2-O-β-D glucopyranoside and cucurbitacin K 2-O-β-Dglucopyranoside (Wang et al. 2007). Besides these, 16-hydroxy 22,23,24,25,26,27hexanorcucurbit-5-en-11, 20-dione3-O-α-L-rhamnopyranosyl-(1 ! 2)-β-Dglucopyranoside, and 2,16-dihydroxy22,23,24,25,26,27 hexanorcucurbit-5-en11,20-dione 2-O-β-D-glucopyranoside have also been reported by Rahman (2013).
Lariciresinol and secoisolariciresinol are the types of lignin which have been
extracted from pumpkin seeds using GC-MS technique (Sicilia et al. 2003).
19.7 Effect of Processing on Characteristics and Chemical
Composition
The pumpkin seed oil extracted after roasting was analyzed using high-performance
liquid chromatography (HPLC) with fluorescence and GC-MS technique to identify
polycyclic aromatic hydrocarbons (PAHs) and volatile compounds, respectively.
Roasting at higher temperatures (150
C) showed the presence of light PAHs
Table 19.7 Phytosterol composition of pumpkin seeds (mg/100 g)
Product
References
Bardaa et al. (2016)
Phillips et al. (2005)
β-Sitosterol
44.405 Æ 0.148
13.1
Campesterol
2.8 Æ 0.014
3.4
Stigma-sterol
2.92 Æ 0.028
ND
Δ
5
-avena-sterol
1.85 Æ 0.014
3.5
Sitostanol
7.75 Æ 0.085
3.5
Campestanol
0.195 Æ 0.007
0.8
Other sterols
NR
241.0
Total phytosterols
NR
265 (252–278)
Cholesterol
0.15 Æ 0.014
NR
24-methylene-cholesterol
0.265 Æ 0.007
NR
Δ
7
-Campesterol
1.275 Æ 0.021
NR
Δ
5–23
-Stigmastadienol
0.045 Æ 0.007
NR
Clerosterol
2.475 Æ 0.035
NR
Δ
5–24
-Stigmastadienol
17.325 Æ 0.063
NR
Δ
7
- Stigmastenol
7.75 Æ 0.085
NR
Δ
7
- Avenasterol
14.51 Æ 0.035
NR
Total sterols (ppm)
2086.5 Æ 19.092
NR
β-Sitosterol (ppm)
959 Æ 21.213
NR
ND not detected, NR not reported
19 Pumpkin (Cucurbita pepo) Seed
483
and was found to be high in oils extracted from husk seeds than in de-hulled/naked
seeds (Nakić et al. 2006). Nawirska-Olszańska et al. (2013) reported that pumpkin
seeds contain cardiac glycosides, terpenoids, and resins. Pumpkin seeds also contain
glycosides viz. cucurbitacin L 2-O-β-D glucopyranoside and cucurbitacin K 2-O-β-Dglucopyranoside (Wang et al. 2007). Besides these, 16-hydroxy 22,23,24,25,26,27hexanorcucurbit-5-en-11, 20-dione3-O-α-L-rhamnopyranosyl-(1 ! 2)-β-Dglucopyranoside, and 2,16-dihydroxy22,23,24,25,26,27 hexanorcucurbit-5-en11,20-dione 2-O-β-D-glucopyranoside have also been reported by Rahman (2013).
Lariciresinol and secoisolariciresinol are the types of lignin which have been
extracted from pumpkin seeds using GC-MS technique (Sicilia et al. 2003).
19.7 Effect of Processing on Characteristics and Chemical
Composition
The pumpkin seed oil extracted after roasting was analyzed using high-performance
liquid chromatography (HPLC) with fluorescence and GC-MS technique to identify
polycyclic aromatic hydrocarbons (PAHs) and volatile compounds, respectively.
Roasting at higher temperatures (150
C) showed the presence of light PAHs
Table 19.7 Phytosterol composition of pumpkin seeds (mg/100 g)
Product
References
Bardaa et al. (2016)
Phillips et al. (2005)
β-Sitosterol
44.405 Æ 0.148
13.1
Campesterol
2.8 Æ 0.014
3.4
Stigma-sterol
2.92 Æ 0.028
ND
Δ
5
-avena-sterol
1.85 Æ 0.014
3.5
Sitostanol
7.75 Æ 0.085
3.5
Campestanol
0.195 Æ 0.007
0.8
Other sterols
NR
241.0
Total phytosterols
NR
265 (252–278)
Cholesterol
0.15 Æ 0.014
NR
24-methylene-cholesterol
0.265 Æ 0.007
NR
Δ
7
-Campesterol
1.275 Æ 0.021
NR
Δ
5–23
-Stigmastadienol
0.045 Æ 0.007
NR
Clerosterol
2.475 Æ 0.035
NR
Δ
5–24
-Stigmastadienol
17.325 Æ 0.063
NR
Δ
7
- Stigmastenol
7.75 Æ 0.085
NR
Δ
7
- Avenasterol
14.51 Æ 0.035
NR
Total sterols (ppm)
2086.5 Æ 19.092
NR
β-Sitosterol (ppm)
959 Æ 21.213
NR
ND not detected, NR not reported
19 Pumpkin (Cucurbita pepo) Seed
483