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53. Rocha C, Gonçalo MP, Teixeira JA (2011) Immobilization of trypsin on spent grains for
whey protein hydrolysis. Process Biochem 46(2):505–511
54. Pedroche J, Yust MM, Lqari H, Giron-Calle J, Vioque J, Alaiz M (2004) Production and
characterization of casein hydrolysates with a high amino acid Fisher’s ratio using
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linkages and thermal stability. Enzyme Microb Technol 40(5):1160–1166
56. Mao Y, Cernigoj U, Zalokar V, Štrancar A, Kulozik U (2017) Production of b-lactoglobulin
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57. Szymanska K, Pietrowska M, Kocurek J, Maresz K, Koreniuk A, Mrowiec-Bialon J, Widłak
P, Magner E, Jarzebski A (2016) Low back-pressure hierarchically structured multichannel
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58. Amorim Fernandes JF, Mcalpine M, Halling P (2005) Operational stability of subtilisin
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61. Nurminen ML, Sipola M, Kaarto H, Pihlanto-Leppälä A, Piilola K, Korpela R,
Tossavainen O, Korhonen H, Vapaatalo H (2000) Alpha-lactorphin lowers blood pressure
measured by radiotelemetry in normotensive and spontaneously hypertensive rats. Life Sci
66(16):1535–1543
62. Sewald N, Jakubke H-D (2009) Peptides: chemistry and biology. Wiley-VCH Verlag GmBH
& Co, KGaA, Weinheim
63. Kumar D, Bhalla TC (2005) Microbial proteases in peptide synthesis: approaches and
applications. Appl Microbiol Biot 68(6):726–736
64. Chen F, Zhang F, Wang A, Li H, Wang Q, Zeng Z, Wang S, Xie T (2010) Recent progress
in the chemo-enzymatic peptide synthesis. Afr J Pharm Pharmacol 4(10):721–730
65. Nuijens T, Quaedflieg PJLM, Jakubke H-D (2012) Hydrolysis and synthesis of peptides. In:
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66. BIOPEP (http://www.uwm.edu.pl/biochemia/index.php/pl/biopep)
67. UniProtKB (http://www.uniprot.org/)
68. ExPASy Peptide Cutter (http://web.expasy.org/peptide_cutter)
69. PoPS algorithm is available at http://mathbio.nimr.mrc.ac.uk/*ffranca/POPS and at the
mirror site http://www.cs.vu.nl/*ibivu/programs/popswww
70. Udenigwe CC (2014) Bioinformatics approaches, prospects and challenges of food bioactive
peptide research. Trends Food Sci Technol 36(2):137–143
71. Fernández A, Zhu Y, FitzGerald RJ, Riera FA (2014) Membrane fractionation of a
b-lactoglobulin tryptic digest: effect of the membrane characteristics. J Chem Technol Biot
89(4):508–515
72. Agyei D, Danquah MK (2011) Industrial-scale manufacturing of pharmaceutical-grade
bioactive peptides. Biotechnol Adv 29(3):272–277
502
A. F. A. Salvado et al.
Garrido SS, Filice M, Monti R (2016) Immobilization of trypsin in lignocellulosic waste
material to produce peptides with bioactive potential from whey protein. Materials 9(5):357
53. Rocha C, Gonçalo MP, Teixeira JA (2011) Immobilization of trypsin on spent grains for
whey protein hydrolysis. Process Biochem 46(2):505–511
54. Pedroche J, Yust MM, Lqari H, Giron-Calle J, Vioque J, Alaiz M (2004) Production and
characterization of casein hydrolysates with a high amino acid Fisher’s ratio using
immobilized proteases. Int Dairy J 14:527–533
55. Pedroche J, Yusta MM, Mateo C, Fernandez-Lafuente R, Giron-Calle J, Alaiz M (2007)
Effect of the support and experimental conditions in the intensity of the multipoint covalent
attachment of proteins on glyoxyl-agarose supports: correlation between enzyme-support
linkages and thermal stability. Enzyme Microb Technol 40(5):1160–1166
56. Mao Y, Cernigoj U, Zalokar V, Štrancar A, Kulozik U (2017) Production of b-lactoglobulin
hydrolysates by monolith based immobilized trypsin reactors. Electrophoresis 38(22–
23):2947–2956
57. Szymanska K, Pietrowska M, Kocurek J, Maresz K, Koreniuk A, Mrowiec-Bialon J, Widłak
P, Magner E, Jarzebski A (2016) Low back-pressure hierarchically structured multichannel
microfluidic bioreactors for rapid protein digestion-proof of concept. Chem Eng J 287:148–
154
58. Amorim Fernandes JF, Mcalpine M, Halling P (2005) Operational stability of subtilisin
CLECs in organic solvents in repeated batch and in continuous operation. Biochem Eng J 24
(1):11–15
59. Guzmán F, Barberis S, Illanes A (2007) Peptide synthesis: chemical or enzymatic. Electron J
Biotechnol 10(2). https://doi.org/10.2225/vol10-issue2-fulltext-13
60. Kimmerlin T, Seebach D (2005) 100 years of peptide synthesis: ligation methods for peptide
and protein synthesis with applications to beta-peptide assemblies. J Pept Res 65(2):229–260
61. Nurminen ML, Sipola M, Kaarto H, Pihlanto-Leppälä A, Piilola K, Korpela R,
Tossavainen O, Korhonen H, Vapaatalo H (2000) Alpha-lactorphin lowers blood pressure
measured by radiotelemetry in normotensive and spontaneously hypertensive rats. Life Sci
66(16):1535–1543
62. Sewald N, Jakubke H-D (2009) Peptides: chemistry and biology. Wiley-VCH Verlag GmBH
& Co, KGaA, Weinheim
63. Kumar D, Bhalla TC (2005) Microbial proteases in peptide synthesis: approaches and
applications. Appl Microbiol Biot 68(6):726–736
64. Chen F, Zhang F, Wang A, Li H, Wang Q, Zeng Z, Wang S, Xie T (2010) Recent progress
in the chemo-enzymatic peptide synthesis. Afr J Pharm Pharmacol 4(10):721–730
65. Nuijens T, Quaedflieg PJLM, Jakubke H-D (2012) Hydrolysis and synthesis of peptides. In:
Drauz K, Gröger H, May O (eds) Enzyme catalysis in organic synthesis. Wiley-VCH Verlag
GmBH & Co. KGaA, Weinheim
66. BIOPEP (http://www.uwm.edu.pl/biochemia/index.php/pl/biopep)
67. UniProtKB (http://www.uniprot.org/)
68. ExPASy Peptide Cutter (http://web.expasy.org/peptide_cutter)
69. PoPS algorithm is available at http://mathbio.nimr.mrc.ac.uk/*ffranca/POPS and at the
mirror site http://www.cs.vu.nl/*ibivu/programs/popswww
70. Udenigwe CC (2014) Bioinformatics approaches, prospects and challenges of food bioactive
peptide research. Trends Food Sci Technol 36(2):137–143
71. Fernández A, Zhu Y, FitzGerald RJ, Riera FA (2014) Membrane fractionation of a
b-lactoglobulin tryptic digest: effect of the membrane characteristics. J Chem Technol Biot
89(4):508–515
72. Agyei D, Danquah MK (2011) Industrial-scale manufacturing of pharmaceutical-grade
bioactive peptides. Biotechnol Adv 29(3):272–277
502
A. F. A. Salvado et al.