Table 4
Dehydrogenase-based biosensors construction and analytical characterization
Enzyme/Source Transduction
a
Analyte
Immobilization/electrode
Electron
Transfer
Detection limit
Linear Range
Sample References
NAD-ADH S.
cerevisiae
Amperometric
(−5 mV)
Ethanol
ADH in MPTS sol-gel (spontaneous
adsorption on the electrode)/AuNPs
self-assembled/Au
Mediated
(NAD
+
via
AuNPs)
20
lM
0.5–2.5 mM
Buffer [163]
NAD-ADH
baker’s yeast
Amperometric
(50 mV)
Ethanol
ADH + NAD
+
+SWCNT + MB + mineral
oil/SWCNT paste
Mediated
(NAD
+
via
MB)
0.1
lM
0.1–4
lM
Buffer [196]
NAD-ADH S
cerevisiae
Amperometric
(40 mV)
Ethanol
ADH + MB crosslinked on MWCNT via
GA/MWCNT-MB
Mediated
(NAD
+
via
MB)
5
lM
5 0
lM–10 mM Buffer [197]
NAD-LDH
rabbit muscle
Amperometric
(600 mV)
Lactate
drop cast LDH + CH + MWCNT/GC
Mediated
(NAD
+
)
0.76
lM
5
–120 lM
Buffer [161]
NAD-GDH
Microorganism
Amperometric
(550 mV)
Glucose
drop cast FePhenTPy
− RGO/drop cast
GDH/Nafion protecting
membrane/CarbonSPE
Mediated
(NAD
+
via
FePhenTPy)
12.02
± 0.6 mg/dL 29–434 mg/dL Blood [198]
FAD-GDH
Aspergillus
terreus
Amperometric
(500 mV)
Glucose
drop cast GDH/GC
Mediated
([Fe(CN)
6 ]
3
−
)
10
lM
5
–30 mM
Buffer [180]
NAD-GluDH
bovine liver
Amperometric
(200 mV)
Glutamate drop cast (GluDH/Pt-PAMAM)
n /CNTs
nanocomposites/GC
Mediated
(NAD
+
)
–
0.2–250
lM
Buffer [162]
NAD-GluDH
beef liver
Amperometric
(100 mV)
Glutamate drop cast MWCNT + CH + MB/drop cast
GluDH + MB + CH + NAD
+
/drop cast
MWCNT + CH + MB/MB-Carbon SPE
Mediated
(NAD
+
via
MB)
3
lM
7.5–105
lM
Buffer [199]
FAD-CDH T.
villosa
Amperometric
(100 mV)
Lactose
drop cast CDH/cross-linking via GA or
PEGDGE/MWCNT/SPE
Direct ET
250 nM
500 nM–
200
lM
Buffer [200]
(continued)
326
T. Monteiro et al.