Table 3 Electrochemical biosensing applications of bilirubin oxidase (BOD) and ascorbate
oxidase (AO x )
Enzyme Analyte
Transducer
Detection
Sample References
BOD
Bilirubin SAM/BOD multilayer Au electrode
Fc–C
mediated
Serum [116]
Bilirubin O 2 electrode with
BOD/BSA/glutaraldehyde
membrane
O 2 depletion Serum [141]
Bilirubin MPTS sol-gel (containing BOD and
AuNPs) Au electrode
Fc mediated Serum [142]
DNAss
PAA-PVP-Os-DNA capture
sequence modified SPE and
DNA-bound BOD probe
O 2 catalytic
reduction
Buffer [122]
Glucose GOx/BOD-MWCNT Au electrode
O 2 catalytic
reduction
Buffer [117]
O 2
BOD-MPA/NA-AuNP Au electrode O 2 catalytic
reduction
SLB
[123]
AOx
Ascorbate O 2 electrode with AOx immobilized
on Teflon membrane
O 2 depletion Fruit
juices
[126]
Ascorbate AOx micelle membrane AGC
electrode
O 2 depletion Fruit
juices
[127]
Ascorbate AOx/eggshell membrane Au
electrode
Ascorbate
catalytic
oxidation
Fruit
juices,
Serum
[128]
Ascorbate AOx/BOD-MWCNT Au electrode
O 2 catalytic
reduction
Buffer [117]
Ascorbate PEDOT-SWCNT/AOx/Nafion Pt
electrode
Ascorbate
catalytic
oxidation
Buffer [114]
Ascorbate Au-deposits graphite electrode
Ascorbate
catalytic
oxidation
Buffer [115]
Paraoxon O 2 electrode with cucumber peel
tissue sandwiched between
Teflon/nylon membrane
O 2 depletion Buffer [130]
AGC aminated glassy carbon; AuNP gold nanoparticles; BSA bovine serum albumin; DNAss DNA
short sequence; GOx glucose oxidase; Fc ferrocene; Fc-C ferrocene carboxylic acid; MPA/NA
3-mercaptopropionic acid/6-amino-2-naphtoic acid; MPTS (3-mercaptopropyl)-trimethoxysilane;
MWCNT multi-walled carbon nanotubes; PAA-PVP-Os copolymer of polyacrylamide and poly (4vinylpyridine)
complexed
with
[Os(2,2′-bipyridine) 2 Cl 2 ]
2+/3+
;
PEDOT
poly
(3,4-ethylenedioxythiophene); SAM self-assembled monolayer; SLB serum-like buffe; SWCNT
single-walled carbon nanotubes
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