• Roles of chalcogens in the O 2 /H
+ competition for biological reducing equivalents; and
• Development of “hybrid” enzymes, or loading of apo-enzymes with synthetic
components for probing the peptide itself.
The major contribution from both chemistry and biology that will guide the
future can be expressed in a single word: knowledge. There is no substitute for it.
Acknowledgements Most of the scientific contributions from the author and coworkers were
produced with funds from the National Science Foundation, most recently grant number
CHE-1665258 and the R. A. Welch Foundation, most recently, A-0924. Special appreciation is
expressed to Professor M. B. Hall, a major contributor to the understanding of Hydrogenase
mechanisms, and a long time collaborator of MYD.
References
1. Vignais PM, Billoud B (2007) Occurrence, classification, and biological function of
hydrogenases: an overview. Chem Rev 107:4206–4272
2. Vignais PM, Billoud B, Meyer J (2001) Classification and phylogeny of hydrogenases.
FEMS Microbiol Rev 25:455–501
3. Copeland A, Nolan M et al (2009) Complete genome sequence of desulfomicrobium
baculatum type strain (X T). Stand Genom Sci 1:29–37
4. Bethel RD, Darensbourg MY (2015) The bioorganometallic Chemistry of hydrogenase.
bioorganometallic Chemistry: applications in drug discovery, biocatalysis, and imaging,
Jaouen G, Salmain M (eds). Wiley-VCH Verlag GmbH & Co, Weinheim, pp 241–272
5. Elsden SR (1981) Hydrogenase 1931–1981. Trends Biochem Sci 6:251–253
6. Stephenson MJ, Stickland LH (1931) Hydrogenases: a bacterial enzyme activating
molecular hydrogen. Biochem J 25:205–214
7. Štrbáňová S (2016) Holding hands with bacteria: the life and work of Marjory Stephenson.
Springer
8. Green DE, Stickland LH (1934) Studies on reversible dehydrogenase systems: The
reversibility of the hydrogenase system of Bact. coli. Biochem J 28:898–900
9. Vincent KA, Cracknell JA, Lenz O et al (2005) Electrocatalytic hydrogen oxidation by an
enzyme at high carbon monoxide or oxygen levels. PNAS 102:16951–16954
10. Pakes WCC, Jollyman WH (1901) The bacterial decomposition of formic acid into carbon
dioxide and hydrogen. J Chem Soc Trans 79:386–391
11. Stephenson M (1949) Bacterial metabolism, 3rd edn. Longmans, Green and Co, London
12. Thauer RK (1998) Biochemistry of methanogenesis: a tribute to Marjory Stephenson.
Microbiology 144:2377–2406
13. Vincent KA, Parkin A, Armstrong FA (2007) Investigating and exploiting the electrocatalytic properties of hydrogenases. Chem Rev 107:4366–4413
14. Conrad R (1996) Soil microorganisms as controllers of atmospheric trace gases (H 2 , CO,
CH 4 , OCS, N 2 O, and NO). Microbiol Rev 60:609–640
15. Goldet G, Wait AF, Cracknell JA et al (2008) Hydrogen production under aerobic conditions
by membrane-bound hydrogenases from Ralstonia species. J Am Chem Soc 130:11106–
11113
16. Moura JJG, Moura I, Huynh BH et al (1982) Unambiguous identification of the nickel EPR
signal in 61Ni-enriched Desulfovibrio gigas hydrogenase. Biochem Biophys Res Commun
108:1388–1393
Organometallic Chemistry Control of Hydrogenases
295
+ competition for biological reducing equivalents; and
• Development of “hybrid” enzymes, or loading of apo-enzymes with synthetic
components for probing the peptide itself.
The major contribution from both chemistry and biology that will guide the
future can be expressed in a single word: knowledge. There is no substitute for it.
Acknowledgements Most of the scientific contributions from the author and coworkers were
produced with funds from the National Science Foundation, most recently grant number
CHE-1665258 and the R. A. Welch Foundation, most recently, A-0924. Special appreciation is
expressed to Professor M. B. Hall, a major contributor to the understanding of Hydrogenase
mechanisms, and a long time collaborator of MYD.
References
1. Vignais PM, Billoud B (2007) Occurrence, classification, and biological function of
hydrogenases: an overview. Chem Rev 107:4206–4272
2. Vignais PM, Billoud B, Meyer J (2001) Classification and phylogeny of hydrogenases.
FEMS Microbiol Rev 25:455–501
3. Copeland A, Nolan M et al (2009) Complete genome sequence of desulfomicrobium
baculatum type strain (X T). Stand Genom Sci 1:29–37
4. Bethel RD, Darensbourg MY (2015) The bioorganometallic Chemistry of hydrogenase.
bioorganometallic Chemistry: applications in drug discovery, biocatalysis, and imaging,
Jaouen G, Salmain M (eds). Wiley-VCH Verlag GmbH & Co, Weinheim, pp 241–272
5. Elsden SR (1981) Hydrogenase 1931–1981. Trends Biochem Sci 6:251–253
6. Stephenson MJ, Stickland LH (1931) Hydrogenases: a bacterial enzyme activating
molecular hydrogen. Biochem J 25:205–214
7. Štrbáňová S (2016) Holding hands with bacteria: the life and work of Marjory Stephenson.
Springer
8. Green DE, Stickland LH (1934) Studies on reversible dehydrogenase systems: The
reversibility of the hydrogenase system of Bact. coli. Biochem J 28:898–900
9. Vincent KA, Cracknell JA, Lenz O et al (2005) Electrocatalytic hydrogen oxidation by an
enzyme at high carbon monoxide or oxygen levels. PNAS 102:16951–16954
10. Pakes WCC, Jollyman WH (1901) The bacterial decomposition of formic acid into carbon
dioxide and hydrogen. J Chem Soc Trans 79:386–391
11. Stephenson M (1949) Bacterial metabolism, 3rd edn. Longmans, Green and Co, London
12. Thauer RK (1998) Biochemistry of methanogenesis: a tribute to Marjory Stephenson.
Microbiology 144:2377–2406
13. Vincent KA, Parkin A, Armstrong FA (2007) Investigating and exploiting the electrocatalytic properties of hydrogenases. Chem Rev 107:4366–4413
14. Conrad R (1996) Soil microorganisms as controllers of atmospheric trace gases (H 2 , CO,
CH 4 , OCS, N 2 O, and NO). Microbiol Rev 60:609–640
15. Goldet G, Wait AF, Cracknell JA et al (2008) Hydrogen production under aerobic conditions
by membrane-bound hydrogenases from Ralstonia species. J Am Chem Soc 130:11106–
11113
16. Moura JJG, Moura I, Huynh BH et al (1982) Unambiguous identification of the nickel EPR
signal in 61Ni-enriched Desulfovibrio gigas hydrogenase. Biochem Biophys Res Commun
108:1388–1393
Organometallic Chemistry Control of Hydrogenases
295