13. Zhang YHP, Evans BR, Mielenz JR, Hopkins RC, Adams MWW (2007) High-yield
hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS ONE 2:
e456
14. Smith PR, Bingham AS, Swartz JR (2012) Generation of hydrogen from NADPH using an
[FeFe] hydrogenase. Int J Hydrogen Energy 37:2977–2983
15. Lu F, Smith FR, Mehta K, Swartz JR (2015) Development of a synthetic pathway to convert
glucose to hydrogen using cell fre extracts. Int J Hydrogen Energy 40:9113–9124
16. Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2007)
Improvement of enzyme activity, stability and selectivity via immobilization technique.
Enzym Microb Technol 40:1451–1463
17. Zadvorny OA, Barrows AM, Zorin NA, Peters JW, Elgren TE (2010) High level of
hydrogen production activity achieved for hydrogenase encapsulated in sol-gel material
doped with carbon nanotubes. J Mater Chem 20:1065–1067
18. Baker SE, Hopkins RC, Blanchette CD, Walsworth VL, Sumbad R, Fischer NO, Kuhn EA,
Coleman M, Chromy BA, Letant SE, Hoeprich PD, Adams MWW, Henderson PT (2009)
Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in
water-soluble nanolipoprotein particles. J Am ChemSoc 131:7508–7509
19. Jordan PC, Patterson DP, Saboda KN, Edwards EJ, Miettinen HM, Basu G, Thielges MC,
Douglas T (2016) Self-assembling biomolecular catalysts for hydrogen production. Nat
Chem 8:179–185
20. Baltazar CSA, Marques MC, Soares CM, DeLacey AM, Pereira IAC, Matias PM (2011)
Nickel-iron-selenium hydrogenases—an overview. Eur J Inorg Chem 948–962
21. Valente FMA, Oliveira ASF, Gnadt N, Pacheco I, Coelho AV, Xavier AV, Teixeira M,
Soares CM, Pereira IAC (2005) Hydrogenases in Desulfovibrio vulgaris Hildenborough:
Structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase.
J Biol Inorg Chem 10:667–682
22. Stein M, Lubitz W (2001) The electronic structure of the catalytic intermediate Ni-C in
[NiFe] and [NiFeSe] hydrogenases. Phys Chem Chem Phys 3:5115–5120
23. Parkin A, Goldet G, Cavazza C, Fontecilla-Camps JC, Armstrong FA (2008) The difference
a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from
Desulfomicrobium baculatum. J Am Chem Soc 130:13410–13416
24. Gutiérrez-Sánchez C, Rüdiger O, Fernández VM et al (2010) Interaction of the active site of
the Ni-Fe-Se hydrogenase from Desulfovibrio vulgaris Hildenborough with carbon
monoxide and oxygen inhibitors. J Biol Inorg Chem 15:1285–1292
25. Maroney MJ, Hondal RJ (2018) Selenium versus sulfur: reversibility of chemical reactions
and resistant to permanent oxidation in proteins and nucleic acids. Free Radical Biol Med
127:228–237
26. Marques MC, Coelho R, De Lacey AL, Pereira IAC, Matias PM (2010) The
three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough: a hydrogenase without a bridging ligand in the active site in its oxidised,
“as-isolated” state. J Mol Biol 396:893–907
27. Marques MC, Tapia C, Gutierrez-Sanz O, Ramos AR, Keller KL, Wall JD, De Lacey AL,
Matias PM, Pereira IAC (2017) The direct role of selenocysteine in NiFeSe hydrogenase
maturation and catalysis. Nat Chem Biol 13:544–550
28. Zacarias S, Temporao A, Del Barrio M, Fourmond V, Leger C, Matias PM, Pereira IAC
(2019) A hydrophillic channel is involved in oxidative inactivation of a [NiFeSe]
hydrogenase. ACS Catal 9:8509–8519
29. Plummer SM, Plummer MA, Merkel P, Hagen M, Biddle J, Waidner L (2016) Using
directed evolution to improve hydrogen production in chimeric hydrogenases from
Clostridia species. Enzym Microb Technol 93:132–141
30. Koo J, Schabel T, liong S, Evitt NH, Swartz JR, (2017) High-throughput screening of
catalytic H 2 production. Angew Chem Int Ed 56:1012–1016
Biological Production of Hydrogen
267
hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS ONE 2:
e456
14. Smith PR, Bingham AS, Swartz JR (2012) Generation of hydrogen from NADPH using an
[FeFe] hydrogenase. Int J Hydrogen Energy 37:2977–2983
15. Lu F, Smith FR, Mehta K, Swartz JR (2015) Development of a synthetic pathway to convert
glucose to hydrogen using cell fre extracts. Int J Hydrogen Energy 40:9113–9124
16. Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2007)
Improvement of enzyme activity, stability and selectivity via immobilization technique.
Enzym Microb Technol 40:1451–1463
17. Zadvorny OA, Barrows AM, Zorin NA, Peters JW, Elgren TE (2010) High level of
hydrogen production activity achieved for hydrogenase encapsulated in sol-gel material
doped with carbon nanotubes. J Mater Chem 20:1065–1067
18. Baker SE, Hopkins RC, Blanchette CD, Walsworth VL, Sumbad R, Fischer NO, Kuhn EA,
Coleman M, Chromy BA, Letant SE, Hoeprich PD, Adams MWW, Henderson PT (2009)
Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in
water-soluble nanolipoprotein particles. J Am ChemSoc 131:7508–7509
19. Jordan PC, Patterson DP, Saboda KN, Edwards EJ, Miettinen HM, Basu G, Thielges MC,
Douglas T (2016) Self-assembling biomolecular catalysts for hydrogen production. Nat
Chem 8:179–185
20. Baltazar CSA, Marques MC, Soares CM, DeLacey AM, Pereira IAC, Matias PM (2011)
Nickel-iron-selenium hydrogenases—an overview. Eur J Inorg Chem 948–962
21. Valente FMA, Oliveira ASF, Gnadt N, Pacheco I, Coelho AV, Xavier AV, Teixeira M,
Soares CM, Pereira IAC (2005) Hydrogenases in Desulfovibrio vulgaris Hildenborough:
Structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase.
J Biol Inorg Chem 10:667–682
22. Stein M, Lubitz W (2001) The electronic structure of the catalytic intermediate Ni-C in
[NiFe] and [NiFeSe] hydrogenases. Phys Chem Chem Phys 3:5115–5120
23. Parkin A, Goldet G, Cavazza C, Fontecilla-Camps JC, Armstrong FA (2008) The difference
a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from
Desulfomicrobium baculatum. J Am Chem Soc 130:13410–13416
24. Gutiérrez-Sánchez C, Rüdiger O, Fernández VM et al (2010) Interaction of the active site of
the Ni-Fe-Se hydrogenase from Desulfovibrio vulgaris Hildenborough with carbon
monoxide and oxygen inhibitors. J Biol Inorg Chem 15:1285–1292
25. Maroney MJ, Hondal RJ (2018) Selenium versus sulfur: reversibility of chemical reactions
and resistant to permanent oxidation in proteins and nucleic acids. Free Radical Biol Med
127:228–237
26. Marques MC, Coelho R, De Lacey AL, Pereira IAC, Matias PM (2010) The
three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough: a hydrogenase without a bridging ligand in the active site in its oxidised,
“as-isolated” state. J Mol Biol 396:893–907
27. Marques MC, Tapia C, Gutierrez-Sanz O, Ramos AR, Keller KL, Wall JD, De Lacey AL,
Matias PM, Pereira IAC (2017) The direct role of selenocysteine in NiFeSe hydrogenase
maturation and catalysis. Nat Chem Biol 13:544–550
28. Zacarias S, Temporao A, Del Barrio M, Fourmond V, Leger C, Matias PM, Pereira IAC
(2019) A hydrophillic channel is involved in oxidative inactivation of a [NiFeSe]
hydrogenase. ACS Catal 9:8509–8519
29. Plummer SM, Plummer MA, Merkel P, Hagen M, Biddle J, Waidner L (2016) Using
directed evolution to improve hydrogen production in chimeric hydrogenases from
Clostridia species. Enzym Microb Technol 93:132–141
30. Koo J, Schabel T, liong S, Evitt NH, Swartz JR, (2017) High-throughput screening of
catalytic H 2 production. Angew Chem Int Ed 56:1012–1016
Biological Production of Hydrogen
267