Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
xi
1 Vaccine Delivery and Immune Response Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1
Andrew Hill, Marie Beitelshees, and Blaine A. Pfeifer
2 Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs)
Using Bacterial Whole Cells as Molecular Probes . . . . . . . . . . . . . . . . . . . . . . . . . . .
9
Sara Siris, Camilla A. Gladstone, Yanping Guo,
Christopher L. Pinder, Robin J. Shattock, Paul F. McKay,
Paul R. Langford, and Fadil A. Bidmos
3 Use of Chlamydial Elementary Bodies as Probes to Isolate
Pathogen-Specific Human Monoclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Christopher L. Pinder, Paul F. McKay, and Robin J. Shattock
4 Computational Antigen Discovery for Eukaryotic
Pathogens Using Vacceed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Stephen J. Goodswen, Paul J. Kennedy, and John T. Ellis
5 Antigen Discovery in Bacterial Panproteomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Daniel Yero, Oscar Conchillo-Sole ´, and Xavier Daura
6 Alphavirus-Based Antigen Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Kenneth Lundstrom
7 Production of Chimeric Hepatitis B Virus Surface Antigens
in Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Mihaela-Olivia Dobrica, Catalin Lazar,
and Norica Branza-Nichita
8 Bioreactor-Based Antigen Production Process Using the Baculovirus
Expression Vector System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Julie Harnischfeger, Lukas K€ aßer, Jan Zitzmann,
Denise Salzig, and Peter Czermak
9 High-Throughput Process Development for the Chromatographic Purification
of Viral Antigens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Shaleem I. Jacob, Spyridon Konstantinidis,
and Daniel G. Bracewell
10 Production of Zika Virus Virus-Like Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Atichat Kuadkitkan, Suwipa Ramphan, Suchin Worawichawong,
Wannapa Sornjai, Nitwara Wikan, and Duncan R. Smith
11 Biosynthesis of Glycoconjugate Virus-like Particles (VLPs). . . . . . . . . . . . . . . . . . . 205
Kathryn K. Oi, Tom A. Kloter, and Timothy G. Keys
12 Upstream and Downstream Processes for Viral Nanoplexes as Vaccines . . . . . . . . 217
Keven Lothert, Gregor Dekevic, Daniel Loewe, Denise Salzig,
Peter Czermak, and Michael W. Wolff
vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
xi
1 Vaccine Delivery and Immune Response Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1
Andrew Hill, Marie Beitelshees, and Blaine A. Pfeifer
2 Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs)
Using Bacterial Whole Cells as Molecular Probes . . . . . . . . . . . . . . . . . . . . . . . . . . .
9
Sara Siris, Camilla A. Gladstone, Yanping Guo,
Christopher L. Pinder, Robin J. Shattock, Paul F. McKay,
Paul R. Langford, and Fadil A. Bidmos
3 Use of Chlamydial Elementary Bodies as Probes to Isolate
Pathogen-Specific Human Monoclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Christopher L. Pinder, Paul F. McKay, and Robin J. Shattock
4 Computational Antigen Discovery for Eukaryotic
Pathogens Using Vacceed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Stephen J. Goodswen, Paul J. Kennedy, and John T. Ellis
5 Antigen Discovery in Bacterial Panproteomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Daniel Yero, Oscar Conchillo-Sole ´, and Xavier Daura
6 Alphavirus-Based Antigen Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Kenneth Lundstrom
7 Production of Chimeric Hepatitis B Virus Surface Antigens
in Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Mihaela-Olivia Dobrica, Catalin Lazar,
and Norica Branza-Nichita
8 Bioreactor-Based Antigen Production Process Using the Baculovirus
Expression Vector System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Julie Harnischfeger, Lukas K€ aßer, Jan Zitzmann,
Denise Salzig, and Peter Czermak
9 High-Throughput Process Development for the Chromatographic Purification
of Viral Antigens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Shaleem I. Jacob, Spyridon Konstantinidis,
and Daniel G. Bracewell
10 Production of Zika Virus Virus-Like Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Atichat Kuadkitkan, Suwipa Ramphan, Suchin Worawichawong,
Wannapa Sornjai, Nitwara Wikan, and Duncan R. Smith
11 Biosynthesis of Glycoconjugate Virus-like Particles (VLPs). . . . . . . . . . . . . . . . . . . 205
Kathryn K. Oi, Tom A. Kloter, and Timothy G. Keys
12 Upstream and Downstream Processes for Viral Nanoplexes as Vaccines . . . . . . . . 217
Keven Lothert, Gregor Dekevic, Daniel Loewe, Denise Salzig,
Peter Czermak, and Michael W. Wolff
vii