50
C, in TRM for 60 min [97]. Afterward, flush with 300 mL
of water in TRM for 5 min.
l
For a full regeneration, and between every filtration run, store
the cassette in 1 M NaOH at 50
C for 40–48 h in a dry-heat
oven [92]. Again, a clean water flux test should be performed,
while a 95% regeneration of the initial water flux should be
achieved [97].
l
If no further runs are performed, store the cassette in 20%
ethanol at 4
C.
3.3.5 Quantification
of Contaminants
dsDNA
l
Let all reagents warm up to RT. Protect Quant-iT™ PicoGreen™ from light.
l
Prepare the buffers as described by the manufacturer. Use the
procedure for 96-well plates. Prepare the buffer for the number
of samples plus 1–2 additional ones, to compensate losses
throughout pipetting. When preparing the TE buffer, consider
Fig. 2 Process flowchart of TFF for the BV purification: (a) shows preparation of system in TRM; (b) the flow
path for the purification process. Abbreviations: P pressure, R reading, W weight
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