Fig. 5 HPLC-AEC analyses of (a) Cps4B and (b) Cps11D reactions. The assay allows the separation and
detection (280 nm) of nucleotide activated donor substrates and released nucleotide products. Substrates and
products were monitored without enzyme (t ¼ 0) and at the indicated time points after the start of the reaction.
The chromatograms demonstrate that Cps4B consumes its donor substrates UDP-GalNAc and UDP-Glc and
simultaneously produces UMP and UDP. Cps11D consumes its donor substrates CDP-Gro and UDP-Gal and
produces CMP and UDP (see Fig. 2)
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Christa Litschko et al.
detection (280 nm) of nucleotide activated donor substrates and released nucleotide products. Substrates and
products were monitored without enzyme (t ¼ 0) and at the indicated time points after the start of the reaction.
The chromatograms demonstrate that Cps4B consumes its donor substrates UDP-GalNAc and UDP-Glc and
simultaneously produces UMP and UDP. Cps11D consumes its donor substrates CDP-Gro and UDP-Gal and
produces CMP and UDP (see Fig. 2)
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Christa Litschko et al.