6. Treier K, Hansen S, Richter C et al (2012)
High-throughput methods for miniaturization
and automation of monoclonal antibody purification
processes.
Biotechnol
Prog
28:723–732
7. Welsh JP, Petroff MG, Rowicki P et al (2014) A
practical strategy for using miniature chromatography columns in a standardized highthroughput workflow for purification development of monoclonal antibodies. Biotechnol
Prog 30:626–635
8. Feliciano J, Berrill A, Ahnfelt M et al (2016)
Evaluating high-throughput scale-down chromatography platforms for increased process
understanding. Eng Life Sci 16:169–178
9. Ła ˛cki KM (2014) High throughput process
development in biomanufacturing. Curr Opin
Chem Eng 6:25–32
10. Konstantinidis S, Goh HY, Martin Bufa ´jer JM
et al (2018) Flexible and accessible automated
operation of miniature chromatography columns on a liquid handling station. Biotechnol
J 13:1700390
11. Li X, De Roo G, Burgers K et al (2012) Selfpacked filter plates: a good alternative for
pre-packed filter plates for developing purification processes for therapeutic proteins. Biotechnol J 7:1269–1276
12. Jacob SI, Khogeer B, Bampos N et al (2017)
Development and application of synthetic
affinity ligands for the purification of ferritinbased influenza antigens. Bioconjug Chem
28:1931–1943
13. El Khoury G, Wang Y, Wang D et al (2013)
Design, synthesis, and assessment of a de novo
affinity adsorbent for the purification of recombinant human erythropoietin. Biotechnol
Bioeng 110:3063–3069
14. Khoury GE, Khogeer B, Chen C et al (2015)
Bespoke affinity ligands for the purification of
therapeutic proteins. Pharmaceut Bioprocess
3:139–152
15. Mcgown EL, Hafeman DG (1998) Multichannel pipettor performance verified by measuring
pathlength of reagent dispensed into a microplate. Anal Biochem 258:155–157
182
Shaleem I. Jacob et al.
High-throughput methods for miniaturization
and automation of monoclonal antibody purification
processes.
Biotechnol
Prog
28:723–732
7. Welsh JP, Petroff MG, Rowicki P et al (2014) A
practical strategy for using miniature chromatography columns in a standardized highthroughput workflow for purification development of monoclonal antibodies. Biotechnol
Prog 30:626–635
8. Feliciano J, Berrill A, Ahnfelt M et al (2016)
Evaluating high-throughput scale-down chromatography platforms for increased process
understanding. Eng Life Sci 16:169–178
9. Ła ˛cki KM (2014) High throughput process
development in biomanufacturing. Curr Opin
Chem Eng 6:25–32
10. Konstantinidis S, Goh HY, Martin Bufa ´jer JM
et al (2018) Flexible and accessible automated
operation of miniature chromatography columns on a liquid handling station. Biotechnol
J 13:1700390
11. Li X, De Roo G, Burgers K et al (2012) Selfpacked filter plates: a good alternative for
pre-packed filter plates for developing purification processes for therapeutic proteins. Biotechnol J 7:1269–1276
12. Jacob SI, Khogeer B, Bampos N et al (2017)
Development and application of synthetic
affinity ligands for the purification of ferritinbased influenza antigens. Bioconjug Chem
28:1931–1943
13. El Khoury G, Wang Y, Wang D et al (2013)
Design, synthesis, and assessment of a de novo
affinity adsorbent for the purification of recombinant human erythropoietin. Biotechnol
Bioeng 110:3063–3069
14. Khoury GE, Khogeer B, Chen C et al (2015)
Bespoke affinity ligands for the purification of
therapeutic proteins. Pharmaceut Bioprocess
3:139–152
15. Mcgown EL, Hafeman DG (1998) Multichannel pipettor performance verified by measuring
pathlength of reagent dispensed into a microplate. Anal Biochem 258:155–157
182
Shaleem I. Jacob et al.