28 Application of Cryogenic Transmission Electron Microscopy for Evaluation
of Vaccine Delivery Carriers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 499
Hui Qian, Yimei Jia, and Michael J. McCluskie
29 Methods to Evaluate Immune Cell Recruitment and Cellular Uptake
and Distribution of Antigen Following Intramuscular Administration
of Vaccine to Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 513
Gerard Agbayani, Felicity C. Stark, Bassel Akache,
and Michael J. McCluskie
30 The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot . . . . . . . . . . . . 525
Bassel Akache and Michael J. McCluskie
31 Measurement of Antigen-Specific IgG Titers by Direct ELISA . . . . . . . . . . . . . . . 537
Bassel Akache, Felicity C. Stark, and Michael J. McCluskie
32 A Method to Evaluate In Vivo CD8
+ T Cell Cytotoxicity
in a Murine Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 549
Felicity C. Stark, Renu Dudani, Gerard Agbayani,
and Michael J. McCluskie
33 Testing Anti-Pneumococcal Antibody Function Using Bacteria
and Primary Neutrophils. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559
Manmeet Bhalla, Shaunna R. Simmons, Essi Y. I. Tchalla,
and Elsa N. Bou Ghanem
34 Viral Infectivity Quantification and Neutralization Assays
Using Laser Force Cytology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 575
Colin G. Hebert, Keya L. Rodrigues, Nicole DiNardo,
and Anna-Barbara Hachmann
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 587
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