From Plant to Patient: Thapsigargin, a Tool for Understanding …
107
125. Jacobsen S, Hansen HS, Jensen B (1987) Synergism between thapsigargin and the phorbol
ester 12-O-tetradecanoylphorbol 13-acetate on the release of [ 14 C]arachidonic acid and
histamine from rat peritoneal mast cells. Biochem Pharmacol 36:621
126. Ohuchi K, Hirasawa N, Takahashi C, Watanabe M, Tsurufuji S, Fujiki H, Suganuma M, Hakii
H, Sugimura T, Christensen SB (1986) Synergistic stimulation of histamine release from rat
peritoneal mast cells by 12-O-tetradecanoylphorbol 13-acetate (TPA)-type and non-TPA-type
tumor promoters. Biochim Biophys Acta Mol Cell Res 887:94
127. Zeilig CE (2014) Intracellular calcium modulation for cancer treatment. US20140065246A1
128. Zeilig CE (2017) Methods for the selective treatment of tumors by calcium-mediated induction
of apoptosis using drugs acting in synergistic combinations. US20170246241A1
129. Szalai P, Parys JB, Bultynck G, Christensen SB, Nissen P, Møller JV, Engedal N (2018)
Nonlinear relationship between ER Ca 2+ depletion versus induction of the unfolded protein
response, autophagy inhibition, and cell death. Cell Calcium 76:48
130. Jackisch C, Hahm HA, Tombal B, McCloskey D, Butash K, Davidson NE, Denmeade SR
(2000) Delayed micromolar elevation in intracellular calcium precedes induction of apoptosis
in thapsigargin-treated breast cancer cells. Clin Cancer Res 6:2844
131. Tombal B, Denmeade SR, Isaacs JT (1999) Assessment and validation of a microinjection
method for kinetic analysis of [Ca 2+ ](i) in individual cells undergoing apoptosis. Cell Calcium
25:19
132. Tombal B, Weeraratna AT, Denmead SR, Isaacs JT (2000) Thapsigargin induces a
calmodulin/calcineurin-dependent apoptotic cascade responsible for the death of prostatic
cancer cells. Prostate 43:303
133. Lin XS, Denmeade SR, Cisek L, Isaacs JT (1997) Mechanism and role of growth arrest
in programmed (apoptotic) death of prostatic cancer cells induced by thapsigargin. Prostate
33:201
134. Zhivotovsky B, Orrenius S (2011) Calcium and cell death mechanisms: a perspective from
the cell death community. Cell Calcium 50:211
135. Bian X, Hughes FM Jr, Huang Y, Cidlowski JA, Putney JW Jr (1997) Roles of cytoplasmic
Ca 2+ and intracellular Ca 2+ stores in induction and suppression of apoptosis in S49 cells. Am
J Physiol 272:C1241
136. Wertz IE, Dixit VM (2000) Characterization of calcium release-activated apoptosis of LNCaP
prostate cancer cells. J Biol Chem 275:11470
137. Skryma R, Mariot P, Bourhis XL, Coppenolle FV, Shuba Y, Vanden Abeele F, Legrand G,
Humez S, Boilly B, Prevarskaya N (2000) Store depletion and store-operated Ca 2+ current in
human prostate cancer LNCaP cells: involvement in apoptosis. J Physiol 527:71
138. Sehgal P, Szalai P, Olesen C, Praetorius HA, Nissen P, Christensen SB, Engedal N, Møller
JV (2017) Inhibition of the sarco/endoplasmic reticulum (ER) Ca 2+ -ATPase by thapsigargin
analogs induces cell death via ER Ca 2+ depletion and the unfolded protein response. J Biol
Chem 292:19656
139. Lindner P, Christensen SB, Nissen P, Møller JV, Engedal N (2020) Cell death induced by the
ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B,
and distinct contributions from unfoldedprotein response components. Cell Commun Signal
18:33
140. Dubois C, Prevarskaya N, Abeele FV (2016) The calcium-signaling toolkit: updates needed.
Biochim Biophys Acta 1863:1337
141. Yamaguchi H, Wang H-G (2004) CHOP is involved in endoplasmic reticulum stress-induced
apoptosis by enhancing DR5 expression in human carcinoma cells. J Biol Chem 279:45495
142. Lu M, Lawrence DA, Marsters S, Acosta-Alvear D, Kimmig P, Mendez AS, Paton AW, Paton
JC, Walter P, Ashkenazi A (2014) Opposing unfolded-protein-response signals converge on
death receptor 5 to control apoptosis. Science 345:98
143. Han D, Lerner AG, Vande Walle L, Upton J-P, Xu W, Hagen A, Backes BJ, Oakes SA,
Papa FR (2009) IRE1α kinase activation modes control alternate endoribonuclease outputs
to determine divergent cell fates. Cell 138:562
107
125. Jacobsen S, Hansen HS, Jensen B (1987) Synergism between thapsigargin and the phorbol
ester 12-O-tetradecanoylphorbol 13-acetate on the release of [ 14 C]arachidonic acid and
histamine from rat peritoneal mast cells. Biochem Pharmacol 36:621
126. Ohuchi K, Hirasawa N, Takahashi C, Watanabe M, Tsurufuji S, Fujiki H, Suganuma M, Hakii
H, Sugimura T, Christensen SB (1986) Synergistic stimulation of histamine release from rat
peritoneal mast cells by 12-O-tetradecanoylphorbol 13-acetate (TPA)-type and non-TPA-type
tumor promoters. Biochim Biophys Acta Mol Cell Res 887:94
127. Zeilig CE (2014) Intracellular calcium modulation for cancer treatment. US20140065246A1
128. Zeilig CE (2017) Methods for the selective treatment of tumors by calcium-mediated induction
of apoptosis using drugs acting in synergistic combinations. US20170246241A1
129. Szalai P, Parys JB, Bultynck G, Christensen SB, Nissen P, Møller JV, Engedal N (2018)
Nonlinear relationship between ER Ca 2+ depletion versus induction of the unfolded protein
response, autophagy inhibition, and cell death. Cell Calcium 76:48
130. Jackisch C, Hahm HA, Tombal B, McCloskey D, Butash K, Davidson NE, Denmeade SR
(2000) Delayed micromolar elevation in intracellular calcium precedes induction of apoptosis
in thapsigargin-treated breast cancer cells. Clin Cancer Res 6:2844
131. Tombal B, Denmeade SR, Isaacs JT (1999) Assessment and validation of a microinjection
method for kinetic analysis of [Ca 2+ ](i) in individual cells undergoing apoptosis. Cell Calcium
25:19
132. Tombal B, Weeraratna AT, Denmead SR, Isaacs JT (2000) Thapsigargin induces a
calmodulin/calcineurin-dependent apoptotic cascade responsible for the death of prostatic
cancer cells. Prostate 43:303
133. Lin XS, Denmeade SR, Cisek L, Isaacs JT (1997) Mechanism and role of growth arrest
in programmed (apoptotic) death of prostatic cancer cells induced by thapsigargin. Prostate
33:201
134. Zhivotovsky B, Orrenius S (2011) Calcium and cell death mechanisms: a perspective from
the cell death community. Cell Calcium 50:211
135. Bian X, Hughes FM Jr, Huang Y, Cidlowski JA, Putney JW Jr (1997) Roles of cytoplasmic
Ca 2+ and intracellular Ca 2+ stores in induction and suppression of apoptosis in S49 cells. Am
J Physiol 272:C1241
136. Wertz IE, Dixit VM (2000) Characterization of calcium release-activated apoptosis of LNCaP
prostate cancer cells. J Biol Chem 275:11470
137. Skryma R, Mariot P, Bourhis XL, Coppenolle FV, Shuba Y, Vanden Abeele F, Legrand G,
Humez S, Boilly B, Prevarskaya N (2000) Store depletion and store-operated Ca 2+ current in
human prostate cancer LNCaP cells: involvement in apoptosis. J Physiol 527:71
138. Sehgal P, Szalai P, Olesen C, Praetorius HA, Nissen P, Christensen SB, Engedal N, Møller
JV (2017) Inhibition of the sarco/endoplasmic reticulum (ER) Ca 2+ -ATPase by thapsigargin
analogs induces cell death via ER Ca 2+ depletion and the unfolded protein response. J Biol
Chem 292:19656
139. Lindner P, Christensen SB, Nissen P, Møller JV, Engedal N (2020) Cell death induced by the
ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B,
and distinct contributions from unfoldedprotein response components. Cell Commun Signal
18:33
140. Dubois C, Prevarskaya N, Abeele FV (2016) The calcium-signaling toolkit: updates needed.
Biochim Biophys Acta 1863:1337
141. Yamaguchi H, Wang H-G (2004) CHOP is involved in endoplasmic reticulum stress-induced
apoptosis by enhancing DR5 expression in human carcinoma cells. J Biol Chem 279:45495
142. Lu M, Lawrence DA, Marsters S, Acosta-Alvear D, Kimmig P, Mendez AS, Paton AW, Paton
JC, Walter P, Ashkenazi A (2014) Opposing unfolded-protein-response signals converge on
death receptor 5 to control apoptosis. Science 345:98
143. Han D, Lerner AG, Vande Walle L, Upton J-P, Xu W, Hagen A, Backes BJ, Oakes SA,
Papa FR (2009) IRE1α kinase activation modes control alternate endoribonuclease outputs
to determine divergent cell fates. Cell 138:562