2. Connect the heater (shown in Fig. 10b) and the motor
(Fig. 10c).
3. Load the material and close the reservoir with the nitrogen
valve (Fig. 10d).
4. Mount the extrusion head in place and turn the machine on.
5. Start the slicing software.
6. Create a new material dataset with the following parameters:
(a) Feed XY: 200 mm/min.
(b) Vol-Disp. (rpm): 100.
(c) Disp. pressure: 10 bar.
(d) Layer thickness: 0.21 mm.
(e) Pattern: 90
.
(f) Strand distance: 1 mm.
(g) Check “Meander.”
(h) See Note 17 for further parameters to be specified.
7. Load the .STL file and select the material dataset just created
for all the layers.
8. Generate the .NC-code and save it.
9. Launch the machine controlling software (Fig. 9) and load the .
NC-code.
10. Stick a stripe of double-sided tape on the building platform.
11. Set the zero point by moving the extrusion head to the desired
manufacturing origin.
12. Set the temperature to 195
C on the temperature controller
and wait for the cartridge to heat up. See Notes 13–16 for
recommendations about the choice of the right temperature
for deposition.
13. After the cartridge has reached the setpoint, allow the polymer
to properly melt for around 15–20 min.
14. In the machine controlling software, click the purge button
until the flow is consistent and smooth.
15. Remove any filament debris from the extruder needle.
16. Press Start on the controlling software.
17. Pay close attention to the first layers as they are those determining the stability of the printout during the process.
18. At the end of the process, let the part cool down and then pour
some ethanol at its base.
19. After some time (i.e., a couple of minutes), the part can be
detached.
20. Dry the scaffold with nitrogen and store it vacuum-sealed in a
dark and dry place.
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