18. At the end of the process, let the part cool down and then pour
some ethanol at its base.
19. After some time (i.e., a couple of minutes), the part can be
detached.
20. Dry the scaffold with nitrogen and store vacuum-sealed in a
dark and dry place.
3.3.3 Bioextrusion
Manufacturing with a SysEng BioScaffolder (Fig. 3a) equipped
with a G22 encapsulation needle (Fig. 4a right) is described as an
example of a screw-based AM system. The following steps need to
be followed for preparation of the system (brief visual instructions
can be found in Supplementary Video 3):
1. Assemble the extrusion head (result of the assembling shown in
Fig. 10a).
Fig. 10 Detailed view of the components for the head assembling procedure with SysEng BioScaffolder: (a) the
print head and the reservoir, (b) the heating element, (c) the motor assembly, (d) the nitrogen pressure valve
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