printing height. Lastly, load the filament (brief visual instructions
can be found in Supplementary Video 1):
1. Mount the 500 μm nozzle (Fig. 4c right) to the extrusion head.
2. Slide the extruder gibs (Fig. 5a) into the head gib slot (Fig. 5b),
and make sure the extruder connector is seated properly
(Fig. 5c).
3. Tighten the thumbscrew to lock the extruder (Fig. 5d).
4. Stick a stripe of double-sided tape on the building platform
(Fig. 5e).
5. Start the controlling software.
Fig. 3 Examples of the three types of extrusion-based AM systems normally used to produce thermoplasticbased scaffolds: (a) SysEng BioScaffolder with a screw extrusion-based head assisted by pressurized
reservoir (bioextrusion example); (b) EnvisionTEC V1.0 Bioplotter, which uses gas pressure-based extrusion
(3DF example); and (c) Hyrel 3D printer with a filament-based head (FDM example)
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can be found in Supplementary Video 1):
1. Mount the 500 μm nozzle (Fig. 4c right) to the extrusion head.
2. Slide the extruder gibs (Fig. 5a) into the head gib slot (Fig. 5b),
and make sure the extruder connector is seated properly
(Fig. 5c).
3. Tighten the thumbscrew to lock the extruder (Fig. 5d).
4. Stick a stripe of double-sided tape on the building platform
(Fig. 5e).
5. Start the controlling software.
Fig. 3 Examples of the three types of extrusion-based AM systems normally used to produce thermoplasticbased scaffolds: (a) SysEng BioScaffolder with a screw extrusion-based head assisted by pressurized
reservoir (bioextrusion example); (b) EnvisionTEC V1.0 Bioplotter, which uses gas pressure-based extrusion
(3DF example); and (c) Hyrel 3D printer with a filament-based head (FDM example)
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Andrea Roberto Calore et al.