Fig. 1 Laponite suspension. Immediately after Laponite addition (0 s) to deionized water, nanosilicate particles
typically result in an opaque solution. After 20 s, Laponite suspension will start to clear, demonstrating
Laponite nanoparticles dispersal. By 50 s, Laponite dispersal achieved, evidenced by a water-clear solution.
Laponite should be left stirring continuously for at least 3 h (see Note 2) to allow complete dispersion of the
nanoparticles in suspension
Fig. 2 Addition of methylcellulose to Laponite-alginate suspension. Immediately after removal of magnetic
stirrer bar, methylcellulose powder can be directly mixed using a sterile spatula (a) addition of methylcellulose
powder deposited on top of the suspension (b). Resultant mixture (c) after at least 1 min of stirring with a
spatula, the mixture can be stored at 4
C overnight to allow methylcellulose homogenous inclusion
Clay Bioinks for Skeletal Regeneration
65
typically result in an opaque solution. After 20 s, Laponite suspension will start to clear, demonstrating
Laponite nanoparticles dispersal. By 50 s, Laponite dispersal achieved, evidenced by a water-clear solution.
Laponite should be left stirring continuously for at least 3 h (see Note 2) to allow complete dispersion of the
nanoparticles in suspension
Fig. 2 Addition of methylcellulose to Laponite-alginate suspension. Immediately after removal of magnetic
stirrer bar, methylcellulose powder can be directly mixed using a sterile spatula (a) addition of methylcellulose
powder deposited on top of the suspension (b). Resultant mixture (c) after at least 1 min of stirring with a
spatula, the mixture can be stored at 4
C overnight to allow methylcellulose homogenous inclusion
Clay Bioinks for Skeletal Regeneration
65