Laser-Driven Behavior of the Nanosystem …
171
Anionic form of the copolymer was obtained via alkaline hydrolysis of Dextrangraft-Polyacrylamide for 30 min by using sodium hydroxide. The fraction of
mers bearing carboxylate groups evaluated by potentiometric titration was equal
to approximately 37% [13].
The synthesis and analysis of internal polymer structure in detail were given a
description in [12, 14].
3 Gold Nanoparticles Synthesis
The AuNPs were synthesized by the chemical reduction of Au precursor (Tetrachloroauric acid). All synthesized polymers play a role of matrices capable to act as
nucleating, capping, and stabilizing agents simultaneously.
0.012 ml of tetrachloroauric acid aqueous solution (C = 0.1 M) was added to
0.5 ml of aqueous polymer solution (C = 1×10
−3 g/cm3) and stirred during 20 min.
Then, 0.047 ml of 0.1 M aqueous solution of sodium borohydride was added. The
final solution was stirred for 30 min. It turned ruby red in color, and thus the formation
of Au NPs was indicated. The reduction process was performed at T = 25 °C. The
size of AuNPs was 1.5–7 nm [11].
4 Synthesis Of Hybrid Nanocomposite Polymer/AuNPs/Ce6
Photosensitizer Chlorine e6 (Ce6) was obtained from Santa Cruz Biotechnology
(USA).
All polymer and polymer/AuNPs composites with Ce6 were prepared ex tempore.
Stock solution of Ce6 (1 mg/ml) was prepared in DMSO. Then, photosensitizer solution was 100-fold diluted in HBSS with constant stirring. The resulting Ce6 solution
was gently mixed with D-g-PAA(PE)/AuNPs or D-g-PAA(PE) and incubated at room
temperature for 5 min before use [10, 11].
5 Experiment
Holographic interferometry technique was used to explore the change of the refractive
index n. The measurement procedure was described in detail in [15]. If a change of
n occurs in the cuvette under the light illumination, then light and dark interference
stripes are observed in the image of the cuvette (Figs. 1 and 2). The larger change of
n provides the greater number of interference stripes. To measure the exact value of
n, we used specially developed technique [15].
Standard cuvettes filled with aqueous solutions of nanocomposites were used as
the samples for investigations. The changes of the refractive index of the solutions
n depending on the time of illumination t irr with a laser (radiation wavelength
was 650 nm, power was 10 mW, beam diameter was ~2 mm) and on the time t rel
171
Anionic form of the copolymer was obtained via alkaline hydrolysis of Dextrangraft-Polyacrylamide for 30 min by using sodium hydroxide. The fraction of
mers bearing carboxylate groups evaluated by potentiometric titration was equal
to approximately 37% [13].
The synthesis and analysis of internal polymer structure in detail were given a
description in [12, 14].
3 Gold Nanoparticles Synthesis
The AuNPs were synthesized by the chemical reduction of Au precursor (Tetrachloroauric acid). All synthesized polymers play a role of matrices capable to act as
nucleating, capping, and stabilizing agents simultaneously.
0.012 ml of tetrachloroauric acid aqueous solution (C = 0.1 M) was added to
0.5 ml of aqueous polymer solution (C = 1×10
−3 g/cm3) and stirred during 20 min.
Then, 0.047 ml of 0.1 M aqueous solution of sodium borohydride was added. The
final solution was stirred for 30 min. It turned ruby red in color, and thus the formation
of Au NPs was indicated. The reduction process was performed at T = 25 °C. The
size of AuNPs was 1.5–7 nm [11].
4 Synthesis Of Hybrid Nanocomposite Polymer/AuNPs/Ce6
Photosensitizer Chlorine e6 (Ce6) was obtained from Santa Cruz Biotechnology
(USA).
All polymer and polymer/AuNPs composites with Ce6 were prepared ex tempore.
Stock solution of Ce6 (1 mg/ml) was prepared in DMSO. Then, photosensitizer solution was 100-fold diluted in HBSS with constant stirring. The resulting Ce6 solution
was gently mixed with D-g-PAA(PE)/AuNPs or D-g-PAA(PE) and incubated at room
temperature for 5 min before use [10, 11].
5 Experiment
Holographic interferometry technique was used to explore the change of the refractive
index n. The measurement procedure was described in detail in [15]. If a change of
n occurs in the cuvette under the light illumination, then light and dark interference
stripes are observed in the image of the cuvette (Figs. 1 and 2). The larger change of
n provides the greater number of interference stripes. To measure the exact value of
n, we used specially developed technique [15].
Standard cuvettes filled with aqueous solutions of nanocomposites were used as
the samples for investigations. The changes of the refractive index of the solutions
n depending on the time of illumination t irr with a laser (radiation wavelength
was 650 nm, power was 10 mW, beam diameter was ~2 mm) and on the time t rel