42
Ie. V. Pylypchuk et al.
Fig. 2.6 Type images of Goryaev’s camera squares with yeast cells: at the beginning of research
(Ã), after a reaction of yeast cells with Fe 3 O 4 /HA NC (b), after a reaction of yeast cells with
Fe 3 O 4 /H£/DR NC (c)
Subsequently, the rate of their proliferation was slowing down (probably due
to a smaller quantity of nutrient material). 3.5 days later their concentration
was ∼10 8 ml −1 . The survivability of yeast cells in the series 1 tests changed
insignificantly and reached ∼ 98–99%.
During testing the suspensions of type 2 control series, a rather active fission was
recorded, as a result of which the yeast concentration was ∼ 6.5·10 7 ml −1 after 18 h,
while after 3.5 days, like in the previous case, it reached ∼ 10 8 ml −1 (Fig. 2.6b). The
survivability of cells, like the in the previous case, at all the research stages of series
2 samples was ∼98–99%. The given data prove the biocompatibility of Fe 3 O 4 /HA
NC in relation to yeast cells in experimental conditions.
By testing of the samples of series 3 suspensions, a considerable inhibition of
cell proliferation was found (Fig. 2.6c). So, the concentration of yeast cells at the
beginning of the experiment was ∼ 3.5·10 7 ml −1 and practically did not change for
16 h; only after 3.5 days, their quantity grew up to ∼ 4·10 7 ml −1 . Over the research
time, the number of dead cells in series 3 samples (Fig. 2.6c) was ∼ 10%.
So, analyzing the results of series 1 and 2 experiments and comparing them with
the series 3 data, it can be concluded that Fe 3 O 4 /H£/DR nanocomposites exert a
cytotoxic effect on Saccharomyces cerevisiae cells and slow down the rate of their
proliferation.
It should be noted that the observed peculiarities of the influence of magnetosensitive Fe3O4/H£/DR NCs on yeast cells are typical of an interaction of the
mentioned cells with doxorubicin in a free form. The obtained results served as a
basis for the elaboration of an efficient, reliable, safe, and relatively inexpensive
methodology of control after the cytotoxic activity of nanocomposites which can be
topical for the use in the development of new magneto-controlled targeted delivery
drugs [39].
Synthesis of Gadolinium-Comprising Magnetosensitive Nanocomposites for
Neutron Capture Therapy The development of scientific approaches to solving
the problem of introduction of magneto-controlled polyfunctional nanocomposites
into neutron capture therapy (NCT) is, certainly, an expedient and topical task
Ie. V. Pylypchuk et al.
Fig. 2.6 Type images of Goryaev’s camera squares with yeast cells: at the beginning of research
(Ã), after a reaction of yeast cells with Fe 3 O 4 /HA NC (b), after a reaction of yeast cells with
Fe 3 O 4 /H£/DR NC (c)
Subsequently, the rate of their proliferation was slowing down (probably due
to a smaller quantity of nutrient material). 3.5 days later their concentration
was ∼10 8 ml −1 . The survivability of yeast cells in the series 1 tests changed
insignificantly and reached ∼ 98–99%.
During testing the suspensions of type 2 control series, a rather active fission was
recorded, as a result of which the yeast concentration was ∼ 6.5·10 7 ml −1 after 18 h,
while after 3.5 days, like in the previous case, it reached ∼ 10 8 ml −1 (Fig. 2.6b). The
survivability of cells, like the in the previous case, at all the research stages of series
2 samples was ∼98–99%. The given data prove the biocompatibility of Fe 3 O 4 /HA
NC in relation to yeast cells in experimental conditions.
By testing of the samples of series 3 suspensions, a considerable inhibition of
cell proliferation was found (Fig. 2.6c). So, the concentration of yeast cells at the
beginning of the experiment was ∼ 3.5·10 7 ml −1 and practically did not change for
16 h; only after 3.5 days, their quantity grew up to ∼ 4·10 7 ml −1 . Over the research
time, the number of dead cells in series 3 samples (Fig. 2.6c) was ∼ 10%.
So, analyzing the results of series 1 and 2 experiments and comparing them with
the series 3 data, it can be concluded that Fe 3 O 4 /H£/DR nanocomposites exert a
cytotoxic effect on Saccharomyces cerevisiae cells and slow down the rate of their
proliferation.
It should be noted that the observed peculiarities of the influence of magnetosensitive Fe3O4/H£/DR NCs on yeast cells are typical of an interaction of the
mentioned cells with doxorubicin in a free form. The obtained results served as a
basis for the elaboration of an efficient, reliable, safe, and relatively inexpensive
methodology of control after the cytotoxic activity of nanocomposites which can be
topical for the use in the development of new magneto-controlled targeted delivery
drugs [39].
Synthesis of Gadolinium-Comprising Magnetosensitive Nanocomposites for
Neutron Capture Therapy The development of scientific approaches to solving
the problem of introduction of magneto-controlled polyfunctional nanocomposites
into neutron capture therapy (NCT) is, certainly, an expedient and topical task