viii
Preface
(anti-EpCAM) for capture of CTCs. Compared to flat PDMS without any surface
structures, these hierarchical substrates exhibited higher capture ability. As indicated by the scanning electron microscope (SEM) and immunofluorescent images,
this enhancement can be partly attributed to the interaction between nanoscale cell
surface components and nanostructures on substrate (topographical interaction). On
the other side, PDMS with hierarchical structures leads to increased surface area,
allowing more anti-EpCAM to be immobilized on the surface, which increases the
number of available sites on the surface for cell adhesion. Furthermore, treating the
substrates with biocompatible reductant glutathione (GSH), 79–85% of the captured
cells can be released with the disulfide bonds being cleaved. The live/dead cell
staining confirmed that the released cells display over 98% cell viability after release.
Therefore, these bio-inspired hierarchical structured and functionalized substrates
can be successfully applied to capture CTCs, as well as release CTCs for subsequent
analysis, providing new prospects for designing cell–material interfaces for advanced
cell-based biomedical studies in the future.
Finally, we would like to thank Prof. Dr. G. Julius Vancso and Prof. Dr. Holger
Schönherr for their guidance and enlightening discussions during preparation of
this book. The authors thank the National Nature Science Foundation of China
(NSFC 51833006) and the Innovation Program of Shanghai Municipal Education
Commission (201701070002E00061) for their financial support.
Shanghai, China
Chuanliang Feng
Xiaoqiu Dou
Yibin Xu
Preface
(anti-EpCAM) for capture of CTCs. Compared to flat PDMS without any surface
structures, these hierarchical substrates exhibited higher capture ability. As indicated by the scanning electron microscope (SEM) and immunofluorescent images,
this enhancement can be partly attributed to the interaction between nanoscale cell
surface components and nanostructures on substrate (topographical interaction). On
the other side, PDMS with hierarchical structures leads to increased surface area,
allowing more anti-EpCAM to be immobilized on the surface, which increases the
number of available sites on the surface for cell adhesion. Furthermore, treating the
substrates with biocompatible reductant glutathione (GSH), 79–85% of the captured
cells can be released with the disulfide bonds being cleaved. The live/dead cell
staining confirmed that the released cells display over 98% cell viability after release.
Therefore, these bio-inspired hierarchical structured and functionalized substrates
can be successfully applied to capture CTCs, as well as release CTCs for subsequent
analysis, providing new prospects for designing cell–material interfaces for advanced
cell-based biomedical studies in the future.
Finally, we would like to thank Prof. Dr. G. Julius Vancso and Prof. Dr. Holger
Schönherr for their guidance and enlightening discussions during preparation of
this book. The authors thank the National Nature Science Foundation of China
(NSFC 51833006) and the Innovation Program of Shanghai Municipal Education
Commission (201701070002E00061) for their financial support.
Shanghai, China
Chuanliang Feng
Xiaoqiu Dou
Yibin Xu