158
8 Nanofabrication on Reactive Block Copolymer Film …
Fig. 8.8 a Schematic of film fabrication: Solvent-treated PS 690 -b-PtBA 1210 films were hydrolyzed
using CF 3 COOH, followed by activation with NHS/EDC. A PEG 500 blocking layer was transferred
on the film by reactive μCP. Finally, dye-labeled BSA molecules were backfilled on the PEG prepatterned film from buffer solution. b Corresponding fluorescence micrograph (and cross-sectional
intensity profile) captured after BSA coupling and thorough rinsing. c AFM height and d FV images
of the cyclohexane-treated PS-b-PtBA polymer films after hydrolysis, reactivation by NHS/EDC and
grafting of BSA. The slight off-set between the height and FV images is the result of the sequential
data acquisition. By convention, the bright areas in the FV images correspond to low pull-off forces
(circular areas have been marked with circles to guide the eyes). Fluorescence emission intensity
histograms for e BSA physisorbed on polystyrene and f BSA covalently coupled on solvent treated
and subsequently activated polymer films
8 Nanofabrication on Reactive Block Copolymer Film …
Fig. 8.8 a Schematic of film fabrication: Solvent-treated PS 690 -b-PtBA 1210 films were hydrolyzed
using CF 3 COOH, followed by activation with NHS/EDC. A PEG 500 blocking layer was transferred
on the film by reactive μCP. Finally, dye-labeled BSA molecules were backfilled on the PEG prepatterned film from buffer solution. b Corresponding fluorescence micrograph (and cross-sectional
intensity profile) captured after BSA coupling and thorough rinsing. c AFM height and d FV images
of the cyclohexane-treated PS-b-PtBA polymer films after hydrolysis, reactivation by NHS/EDC and
grafting of BSA. The slight off-set between the height and FV images is the result of the sequential
data acquisition. By convention, the bright areas in the FV images correspond to low pull-off forces
(circular areas have been marked with circles to guide the eyes). Fluorescence emission intensity
histograms for e BSA physisorbed on polystyrene and f BSA covalently coupled on solvent treated
and subsequently activated polymer films