122
6 Fabrication of Robust Biomolecular Patterns …
The fluorescence emission of these dyes was recorded with photomultiplier tubes
(Hamamatsu R6357) after spectral filtering with a 500–550 nm bandpass filter and a
650 nm long-pass filter for fluoresceinamine and Cy5-labeled DNA and BSA-Alexa
Fluor
® 594, respectively. Images were acquired with maximum pinhole diameters.
Atomic Force Microscopy (AFM). The contact mode AFM measurements were
carried out with a NanoScope X multimode AFM (Digital Instruments/Veeco,
Santa Barbara, CA) using a 100 μm scanner and microfabricated silicon nitride
tips/cantilevers (Model NP, Veeco Nano Probe, Veeco, Santa Barbara, CA) in ambient
atmosphere (ca. 30% relative humidity, 24 °C temperature).
References
1. Singhvi R, Kumar A, Lopez GP, Stephanopoulos GN, Wang DI, Whitesides GM (1994)
Engineering cell-shape and function. Science 264:696–698
2. MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput
function determination. Science 289:1760–1763
3. Brack HP, Padeste C, Slaski M, Alkan S, Solak HH (2004) Preparation of micro- and
nanopatterns of polymer chains grafted onto flexible polymer substrates. J Am Chem Soc
126:1004–1005
4. Lussi JW, Michel R, Reviakine I, Falconnet D, Goessl A, Csucs G, Hubbell JA, Textor M
(2004) A novel generic platform for chemical patterning of surface. Prog Surf Sci 76:55–69
5. Wang C, Zhang Y (2005) Protein micropatterning via self-assembly of nanoparticles. Adv
Mater 17:150–153
6. Kumar A, Whitesides GM (2002) Features of gold having micrometer to centimeter dimensions
can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol
ink followed by chemical etching. Appl Phys Lett 63:2002–2004
7. Crooks RM, Ricco AJ (1998) New organic materials suitable for use in chemical sensor arrays.
Acc Chem Res 31:219–227
8. Quake SR, Scherer A (2000) From micro- to nanofabrication with soft materials. Science
290:1536–1540
9. Renault JP, Bernard A, Juncker D, Michel B, Bosshare HR, Delamarche E (2002) Fabricating
microarrays of functional proteins using affinity contact printing. Angew Chem Int Ed 41:2320–
2323
10. Anzai J, Kobayashi Y, Nakamura N, Nishimura M, Hoshi T (1999) Layer-by-layer construction
of multilayer thin films composed of avidin and biotin-labeled poly(amine)s. Langmuir 15:221–
226
11. Pathak S, Singh AK, McElhanon JR, Dentinger PM (2004) Dendrimer-activated surfaces for
high density and high activity protein chip applications. Langmuir 20:6075–6079
12. Degenhart GH, Dordi B, Schönherr H, Vancso GJ (2004) Micro- and nanofabrication of robust
reactive arrays based on the covalent coupling of dendrimers to activated monolayers. Langmuir
20:6216–6264
13. Xia YN, Mrksich M, Kim E, Whitesides GM (1995) Microcontact printing of octadecylsiloxane
on the surface of silicon dioxide and its application in microfabrication. J Am Chem Soc
117:9576–9577
14. Knoll W, Matsuzawa M, Offenhäusser A, Rühe J (1996) Tailoring of surfaces with ultrathin
layers for controlled binding of biopolymers and adhesion and guidance of cells. Isr J Chem
36:357–369
15. Wells M, Crooks RM (1996) Interactions between organized, surface-confined monolayers and
vapor-phase probe molecules.10. Preparation and properties of chemically sensitive dendrimer
surfaces. J Am Chem Soc 118:3988–3989
6 Fabrication of Robust Biomolecular Patterns …
The fluorescence emission of these dyes was recorded with photomultiplier tubes
(Hamamatsu R6357) after spectral filtering with a 500–550 nm bandpass filter and a
650 nm long-pass filter for fluoresceinamine and Cy5-labeled DNA and BSA-Alexa
Fluor
® 594, respectively. Images were acquired with maximum pinhole diameters.
Atomic Force Microscopy (AFM). The contact mode AFM measurements were
carried out with a NanoScope X multimode AFM (Digital Instruments/Veeco,
Santa Barbara, CA) using a 100 μm scanner and microfabricated silicon nitride
tips/cantilevers (Model NP, Veeco Nano Probe, Veeco, Santa Barbara, CA) in ambient
atmosphere (ca. 30% relative humidity, 24 °C temperature).
References
1. Singhvi R, Kumar A, Lopez GP, Stephanopoulos GN, Wang DI, Whitesides GM (1994)
Engineering cell-shape and function. Science 264:696–698
2. MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput
function determination. Science 289:1760–1763
3. Brack HP, Padeste C, Slaski M, Alkan S, Solak HH (2004) Preparation of micro- and
nanopatterns of polymer chains grafted onto flexible polymer substrates. J Am Chem Soc
126:1004–1005
4. Lussi JW, Michel R, Reviakine I, Falconnet D, Goessl A, Csucs G, Hubbell JA, Textor M
(2004) A novel generic platform for chemical patterning of surface. Prog Surf Sci 76:55–69
5. Wang C, Zhang Y (2005) Protein micropatterning via self-assembly of nanoparticles. Adv
Mater 17:150–153
6. Kumar A, Whitesides GM (2002) Features of gold having micrometer to centimeter dimensions
can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol
ink followed by chemical etching. Appl Phys Lett 63:2002–2004
7. Crooks RM, Ricco AJ (1998) New organic materials suitable for use in chemical sensor arrays.
Acc Chem Res 31:219–227
8. Quake SR, Scherer A (2000) From micro- to nanofabrication with soft materials. Science
290:1536–1540
9. Renault JP, Bernard A, Juncker D, Michel B, Bosshare HR, Delamarche E (2002) Fabricating
microarrays of functional proteins using affinity contact printing. Angew Chem Int Ed 41:2320–
2323
10. Anzai J, Kobayashi Y, Nakamura N, Nishimura M, Hoshi T (1999) Layer-by-layer construction
of multilayer thin films composed of avidin and biotin-labeled poly(amine)s. Langmuir 15:221–
226
11. Pathak S, Singh AK, McElhanon JR, Dentinger PM (2004) Dendrimer-activated surfaces for
high density and high activity protein chip applications. Langmuir 20:6075–6079
12. Degenhart GH, Dordi B, Schönherr H, Vancso GJ (2004) Micro- and nanofabrication of robust
reactive arrays based on the covalent coupling of dendrimers to activated monolayers. Langmuir
20:6216–6264
13. Xia YN, Mrksich M, Kim E, Whitesides GM (1995) Microcontact printing of octadecylsiloxane
on the surface of silicon dioxide and its application in microfabrication. J Am Chem Soc
117:9576–9577
14. Knoll W, Matsuzawa M, Offenhäusser A, Rühe J (1996) Tailoring of surfaces with ultrathin
layers for controlled binding of biopolymers and adhesion and guidance of cells. Isr J Chem
36:357–369
15. Wells M, Crooks RM (1996) Interactions between organized, surface-confined monolayers and
vapor-phase probe molecules.10. Preparation and properties of chemically sensitive dendrimer
surfaces. J Am Chem Soc 118:3988–3989