3.1 History of DET-Type Bioelectrocatalysis
61
Table 3.1
(continued)
Enzyme group
Enzyme name (source)
Catalytic and redox center(s)* (subunit structure)
Reaction
(half-wave
potential of the
catalytic wave)
Electrode
Refs. PDB
[NiFe] hydrogenase
(Desulfovibrio vulgaris
Miyazaki
F)
(NiFe, 4Fe4S, 3Fe4S, 4Fe4S)×2 [a
2 b
2 heterotetramer]
2H +
+ 2e −
H2
(−0.5 V vs.
Ag|AgCl, pH 5)
p-PDA-modified
KBE
[25] 1h2r
[NiFe] hydrogenase
(Hydrogenovibrio marinus)
(NiFe, 4Fe4S, 3Fe4S, 4Fe4S)×2 [a
2 b
2 heterotetramer]
H2 – 2e −
(−0.4 V
vs. Ag|AgCl, pH
5.5)
KBE
[26] 3ayx
[FeFe] hydrogenase
(Desulfovibrio desulfuricans)
(FeFe, 4Fe4S, 4Fe4S, 4Fe4S)×2 [a
2 b
2 heterotetramer]
2H +
+ 2e −
H2
(−0.3 V vs. SHE,
pH 6)
EPGE
[27]
NAD-reducing [NiFe]
hydrogenase (Hydrogenophilus
thermoluteolus)
NiFe, 4Fe4S, 4Fe4S, 2Fe2S, 4Fe4S, FMN (non-covalent)
[heterodimer]
2H +
+ 2e −
H2, NAD +
+ 2e −
NADH
(−0.6 V vs.
Ag|AgCl, pH 7)
[heterodimer]
KBE
[28] 5xFA
NAD-reducing formate
dehydrogenase
(Methylobacterium extorquens
AM1)
W-pterin, 4Fe4S, 4Fe4S, 2Fe2S, 4Fe4S, FMN (non-covalent)
[heterodimer]
CO
2
+ 2e −
COO −
, NAD +
+
2e −
NADH
(−0.55 V vs.
Ag|AgCl, pH 7)
AuNP/KBE
[29]
NAD-reducing formate
dehydrogenase 1
(Syntrophobacter fumaroxidans)
W-pterin, 2Fe2S´4, 4Fe4S´4? [heterotrimer]
CO
2
+ 2e −
COO −
, (−0.35 V
vs. SHE, pH 6.4)
EPGE
[30]
(continued)