60
3 Fundamentals of DET-Type Bioelectrocatalysis
Table 3.1
Performance of DET-type bioelectrocatalysis of several redox enzymes
Enzyme group
Enzyme name (source)
Catalytic and redox center(s)* (subunit structure)
Reaction
(half-wave
potential of the
catalytic wave)
Electrode
Refs. PDB
Copper-containing enzyme
Laccase (Pycnoporus
cinnabarinus)
Type II/III 3Cu cluster, Type I Cu (blue copper) [monomer]
O
2
+ 4e −
(0.45 V
vs. SCE, pH 3)
EPGE
[7]
2XYB
O
2
+ 4e −
(0.7 V
vs. SHE, pH 4)
Anthracene-modified EPGE
[18]
Bilirubin oxidase (Myrothecium
verrucaria)
Type II/III 3Cu cluster, Type I Cu (blue copper) [monomer]
O
2
+ 4e −
(0.55 V
vs. Ag|AgCl, pH
7)
MWCNT
[19] 2XLL
O
2
+ 4e −
(0.4 V
vs. Ag|AgCl, pH
7)
AuNP
[20]
O
2
+ 4e −
(0.5 V
vs. Ag|AgCl, pH
5)
MgO-templated porous carbon [21]
Laccase (Pycnoporus
cinnabarinus)
Type II/III 3Cu cluster, Type I Cu (blue copper) [monomer]
O
2
+ 4e −
(0.45 V
vs. SCE, pH 3)
EPGE
[22] 2XYB
Copper efflux oxidase
(Escherichia coli)
Type II/III 3Cu cluster, Type I Cu (blue copper), Cu 2+
[monomer] O
2
+ 4e −
(0.3 V
vs. Ag|AgCl, pH
5)
KBE
[7]
4NER
Nitrite reductase (Alcaligenes
faecalis)
(Type II Cu, Type I Cu)×2 [homodimer]
NO
2
−
+ e −
(0.1 V (?) vs.
SHE, pH 7)
EPGE
[23] 4YSC
FeS-based electron-relay-system-containing enzyme
[NiFe] hydrogenase
(Chromatium vinosum)
(NiFe, 4Fe4S (proximal), 3Fe4S (medial), 4Fe4S (distal))´2
[a
2 b
2 heterotetramer]
2H +
+ 2e −
H2
(−0.35 V vs.
SHE, pH 6)
PGE (edge)
[24]
(continued)