5.4 Protein Surface Modification
101
Fig. 5.6 A Cartoon representation showing the secondary structure of S362CMoBOD variant
and its attachment to the maleimide-modified surface. The location of the engineered Cys residue
on the surface is highlighted in blue. B, C Bioelectrocatalytic waves of O 2 reduction at B a
S362CMoBOD- and C wild type MoBOD-modified electrodes by covalent attachment through
maleimide on GC/MWCNT electrode and by drop-casting 3 µL of the enzyme solution on the
electrode. The black and green curves show DET-type bioelectrocatalysis in deoxygenated and
O 2 -saturated solutions, respectively. The blue and red curves show MET-type bioelectrocatalysis in
deoxygenated and O 2 -saturated solutions, respectively, containing 0.3 mM ABTS at the same electrodes. Reproduced from Ref. [46] copyright (2019) (with minor modification) from The American
Chemical Society
to contain a stretch of six consecutive His residues at the N- or C-terminus were
attached via their affinities to the Ni–NTA moiety. Successful construction of an
oriented monolayer of cyt c oxidase on an Au electrode surface was demonstrated,
and DET-type bioelectrocatalysis of cyt c oxidase was confirmed [48, 49].
References
1. Adachi T, Kitazumi Y, Shirai O, Kano K (2020) Direct electron transfer-type bioelectrocatalysis
of redox enzymes at nanostructured electrodes. Catalysts 10:236
2. Sakai K, Kitazumi Y, Shirai O, Kano K (2018) Nanostructured porous electrodes by the anodization of gold for an application as scaffolds in direct-electron-transfer-type bioelectrocatalysis.
Anal Sci 34:1317–1322
3. Xia H, Kitazumi Y, Shirai O, Kano K (2017) Direct electron transfer-type bioelectrocatalysis
of peroxidase at mesoporous carbon electrodes and its application for glucose determination
based on bienzyme system. Anal Sci 33:839
4. Takahashi Y, Wanibuchi M, Kitazumi Y, Shirai O, Kano K (2019) Improved direct electron
transfer-type bioelectrocatalysis of bilirubin oxidase using porous gold electrodes. J Electroanal
Chem 843:47–53
5. Wanibuchi M, Takahashi Y, Kitazumi Y, Shirai O, Kano K (2020) Significance of nanostructures of carbon materials for direct-electron-transfer-type bioelectrocatalysis of bilirubin
oxidase. Electrochemistry (in press). https://doi.org/10.5796/electrochemistry.5720-64063
6. Mazurenko I, de Poulpiquet A, Lojou E (2017) Recent developments in high surface area
bioelectrodes for enzymatic fuel cells. Curr Opin Electrochem 5:74–84
7. Sugimoto Y, Kitazumi Y, Shirai O, Kano K (2017) Effects of mesoporous structures on direct
electron transfer-type bioelectrocatalysis: facts and simulation on a three-dimensional model
of random orientation of enzymes. Electrochemistry 85:82–87
101
Fig. 5.6 A Cartoon representation showing the secondary structure of S362CMoBOD variant
and its attachment to the maleimide-modified surface. The location of the engineered Cys residue
on the surface is highlighted in blue. B, C Bioelectrocatalytic waves of O 2 reduction at B a
S362CMoBOD- and C wild type MoBOD-modified electrodes by covalent attachment through
maleimide on GC/MWCNT electrode and by drop-casting 3 µL of the enzyme solution on the
electrode. The black and green curves show DET-type bioelectrocatalysis in deoxygenated and
O 2 -saturated solutions, respectively. The blue and red curves show MET-type bioelectrocatalysis in
deoxygenated and O 2 -saturated solutions, respectively, containing 0.3 mM ABTS at the same electrodes. Reproduced from Ref. [46] copyright (2019) (with minor modification) from The American
Chemical Society
to contain a stretch of six consecutive His residues at the N- or C-terminus were
attached via their affinities to the Ni–NTA moiety. Successful construction of an
oriented monolayer of cyt c oxidase on an Au electrode surface was demonstrated,
and DET-type bioelectrocatalysis of cyt c oxidase was confirmed [48, 49].
References
1. Adachi T, Kitazumi Y, Shirai O, Kano K (2020) Direct electron transfer-type bioelectrocatalysis
of redox enzymes at nanostructured electrodes. Catalysts 10:236
2. Sakai K, Kitazumi Y, Shirai O, Kano K (2018) Nanostructured porous electrodes by the anodization of gold for an application as scaffolds in direct-electron-transfer-type bioelectrocatalysis.
Anal Sci 34:1317–1322
3. Xia H, Kitazumi Y, Shirai O, Kano K (2017) Direct electron transfer-type bioelectrocatalysis
of peroxidase at mesoporous carbon electrodes and its application for glucose determination
based on bienzyme system. Anal Sci 33:839
4. Takahashi Y, Wanibuchi M, Kitazumi Y, Shirai O, Kano K (2019) Improved direct electron
transfer-type bioelectrocatalysis of bilirubin oxidase using porous gold electrodes. J Electroanal
Chem 843:47–53
5. Wanibuchi M, Takahashi Y, Kitazumi Y, Shirai O, Kano K (2020) Significance of nanostructures of carbon materials for direct-electron-transfer-type bioelectrocatalysis of bilirubin
oxidase. Electrochemistry (in press). https://doi.org/10.5796/electrochemistry.5720-64063
6. Mazurenko I, de Poulpiquet A, Lojou E (2017) Recent developments in high surface area
bioelectrodes for enzymatic fuel cells. Curr Opin Electrochem 5:74–84
7. Sugimoto Y, Kitazumi Y, Shirai O, Kano K (2017) Effects of mesoporous structures on direct
electron transfer-type bioelectrocatalysis: facts and simulation on a three-dimensional model
of random orientation of enzymes. Electrochemistry 85:82–87