Chapter 28
Chemically Defined, Clinical-Grade Cryopreservation
of Human Adipose Stem Cells
Melany Lo ´ pez and Ali Eroglu
Abstract
Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily
harvested in large quantities through a clinically safe liposuction procedure. ASCs do not induce immunogenic reactions and rather exert immunosuppressive effects. Therefore, they can be used for both autologous and allogeneic transplantations. They hold great promise for cell-based therapies and tissue
engineering. A prerequisite to the realization of this promise is the development of successful cryopreservation methods for ASCs. In this chapter, we describe a xeno-free- and chemically defined cryopreservation
protocol, which can be used for various clinical applications of ASCs.
Key words Cryopreservation, Human adipose stem cells, Clinical-grade cryopreservation
1 Introduction
Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily harvested in large quantities
through a clinically safe liposuction procedure [1, 2]. Moreover,
compared to bone marrow-derived stem cells, ASCs can be
expanded in vitro for longer periods of time [3–5] without losing
their plasticity to differentiate into various lineages including osteogenic, chondrogenic, adipogenic, and neurogenic [5–9]. Published data suggest that ASCs do not induce immunogenic
reactions and rather exert immunosuppressive effects [10–
13]. Therefore, they can be used for both autologous and allogeneic transplantation. In fact, it has already been demonstrated that
ASCs exercise beneficial effects on the healing of different diseases
including acute myocardial infarction, peripheral vascular disease,
bony tissue defects, recurrent Crohn’s fistulae, and chronic skin
wounds [1, 14–18]. These features of ASCs hold great promise for
cell-based therapies and tissue engineering. A prerequisite to the
realization of this promise is the development of successful cryopreservation methods for ASCs. While currently used
Willem F. Wolkers and Harrie ¨ tte Oldenhof (eds.), Cryopreservation and Freeze-Drying Protocols, Methods in Molecular Biology,
vol. 2180, https://doi.org/10.1007/978-1-0716-0783-1_28, © Springer Science+Business Media, LLC, part of Springer Nature 2021
555
Chemically Defined, Clinical-Grade Cryopreservation
of Human Adipose Stem Cells
Melany Lo ´ pez and Ali Eroglu
Abstract
Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily
harvested in large quantities through a clinically safe liposuction procedure. ASCs do not induce immunogenic reactions and rather exert immunosuppressive effects. Therefore, they can be used for both autologous and allogeneic transplantations. They hold great promise for cell-based therapies and tissue
engineering. A prerequisite to the realization of this promise is the development of successful cryopreservation methods for ASCs. In this chapter, we describe a xeno-free- and chemically defined cryopreservation
protocol, which can be used for various clinical applications of ASCs.
Key words Cryopreservation, Human adipose stem cells, Clinical-grade cryopreservation
1 Introduction
Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily harvested in large quantities
through a clinically safe liposuction procedure [1, 2]. Moreover,
compared to bone marrow-derived stem cells, ASCs can be
expanded in vitro for longer periods of time [3–5] without losing
their plasticity to differentiate into various lineages including osteogenic, chondrogenic, adipogenic, and neurogenic [5–9]. Published data suggest that ASCs do not induce immunogenic
reactions and rather exert immunosuppressive effects [10–
13]. Therefore, they can be used for both autologous and allogeneic transplantation. In fact, it has already been demonstrated that
ASCs exercise beneficial effects on the healing of different diseases
including acute myocardial infarction, peripheral vascular disease,
bony tissue defects, recurrent Crohn’s fistulae, and chronic skin
wounds [1, 14–18]. These features of ASCs hold great promise for
cell-based therapies and tissue engineering. A prerequisite to the
realization of this promise is the development of successful cryopreservation methods for ASCs. While currently used
Willem F. Wolkers and Harrie ¨ tte Oldenhof (eds.), Cryopreservation and Freeze-Drying Protocols, Methods in Molecular Biology,
vol. 2180, https://doi.org/10.1007/978-1-0716-0783-1_28, © Springer Science+Business Media, LLC, part of Springer Nature 2021
555