7. Hang the washing solutions on the upper hook of the raised IV
pole. Using aseptic technique, connect the washing solutions
to the disposable:
(a) Spike the 12% NaCl bag to the blue-striped line.
(b) Spike the saline/glucose bag to the yellow-striped line.
(c) Connect the Luer lock connector of the storage solution
bag to the orange-striped line and crack the breakable seal.
8. Sterilely connect the red-striped line to the tubing of the
thawed RBC bag. Leave the weld closed.
9. Place the thawed RBC unit on the shaker, using the magnetic
pins with the ports toward the front of the shaker.
10. Verify that all colored tubing segments are loaded properly in
the appropriate valves and press YES. After doing this, the
machine guides the user through a set of verification questions
to ensure the proper loading of the disposable set. Mistakes in
loading the disposable set can be corrected in this stage.
Improper installation of the disposable set may result in product damage.
11. Upon the message “Sterile Connect RBC bag to RED striped
tubing,” open the weld between RBC bag and disposable set.
Check the weld. If the weld is not correct, reconnect the unit
and mark that the unit is not sterilely processed and has a 24-h
post-wash storage limit.
12. The ACP215 prompts the operator to enter his/her initials (see
Note 2).
13. Upon the message “Press modify to set parameters,” press
“Modify” and enter the weight of the RBC unit using the
YES/UP or NO/DOWN arrows. Press “Modify.” The
ACP215 will display the following message: “Thawed unit
hematocrit.” Use the YES/UP or NO/DOWN arrows to
adjust the setting to 30% if RBCs were frozen without glycerol
removal or to 60% in case glycerol was removed before
freezing.
14. Press START to begin the deglycerolization process.
15. After about 15 min, the ACP215 will evaluate the integrity of
the connection between the RBC bag and the disposable by
holding and monitoring a negative pressure for a period of
time. The message “Sterile connection check in progress” will
be displayed. A steady pressure level indicates an intact weld,
and the machine will continue its operation as normal. If the
test fails, the intactness of the weld should be visually inspected.
If the weld is intact, press START to continue the procedure. If
the weld is incorrect and NO is pressed, a statement will be
printed on the print out of the procedure stating that the shelf
life of the blood product is limited to 24 h (see Note 6).
Cryopreservation of Red Blood Cells
531
pole. Using aseptic technique, connect the washing solutions
to the disposable:
(a) Spike the 12% NaCl bag to the blue-striped line.
(b) Spike the saline/glucose bag to the yellow-striped line.
(c) Connect the Luer lock connector of the storage solution
bag to the orange-striped line and crack the breakable seal.
8. Sterilely connect the red-striped line to the tubing of the
thawed RBC bag. Leave the weld closed.
9. Place the thawed RBC unit on the shaker, using the magnetic
pins with the ports toward the front of the shaker.
10. Verify that all colored tubing segments are loaded properly in
the appropriate valves and press YES. After doing this, the
machine guides the user through a set of verification questions
to ensure the proper loading of the disposable set. Mistakes in
loading the disposable set can be corrected in this stage.
Improper installation of the disposable set may result in product damage.
11. Upon the message “Sterile Connect RBC bag to RED striped
tubing,” open the weld between RBC bag and disposable set.
Check the weld. If the weld is not correct, reconnect the unit
and mark that the unit is not sterilely processed and has a 24-h
post-wash storage limit.
12. The ACP215 prompts the operator to enter his/her initials (see
Note 2).
13. Upon the message “Press modify to set parameters,” press
“Modify” and enter the weight of the RBC unit using the
YES/UP or NO/DOWN arrows. Press “Modify.” The
ACP215 will display the following message: “Thawed unit
hematocrit.” Use the YES/UP or NO/DOWN arrows to
adjust the setting to 30% if RBCs were frozen without glycerol
removal or to 60% in case glycerol was removed before
freezing.
14. Press START to begin the deglycerolization process.
15. After about 15 min, the ACP215 will evaluate the integrity of
the connection between the RBC bag and the disposable by
holding and monitoring a negative pressure for a period of
time. The message “Sterile connection check in progress” will
be displayed. A steady pressure level indicates an intact weld,
and the machine will continue its operation as normal. If the
test fails, the intactness of the weld should be visually inspected.
If the weld is intact, press START to continue the procedure. If
the weld is incorrect and NO is pressed, a statement will be
printed on the print out of the procedure stating that the shelf
life of the blood product is limited to 24 h (see Note 6).
Cryopreservation of Red Blood Cells
531