osmolarity decrease rate exceeding 500 mOsm/kg.min. After the
second dilution, the diluted cells are transferred to the bowl where
the washing procedure in the bowl, using five washing steps with
0.9% NaCl/0.2% glucose solution, begins. Effluent exiting the
bowl will be collected in the waste bag. The line sensor monitors
the free hemoglobin level and cell spillage in the effluent. At the
completion of the washing procedure, the cells are washed once
with additive solution and finally resuspended in the additive solution. The deglycerolized RBCs are transferred from the washing
bowl into the blood product bag of the disposable set. The entire
deglycerolization procedure takes about 60–70 min.
3.2.1 Thawing the Unit
1. Remove the frozen unit from the freezer and from the
cardboard box.
2. Place the unit with the overwrap bag in a water bath maintained
at 40
C.
3. After 20 min, remove the overwrap and unfold the blood bag.
4. Monitor the temperature of the blood bag using a noncontact
thermometer. The temperature of the blood bag should be
between 25 and 30
C. Warm the unit if needed, taking care
not to wet the ports of the bag.
3.2.2 Deglycerolization
Procedure
1. Power-on the ACP215. The ACP215 automatically performs a
series of internal system checks.
2. Select the deglycerolization protocol.
3. Open the deglycerolization disposable set and inspect it for
visible defects.
4. Install the disposable set according to the manufacturer’s
instructions.
5. After loading the disposable set, the display posts the message
asking the operator if the Line Sensor Calibration Check is
needed. It is recommended to perform this check each day of
use. The line sensor is calibrated using the filters labeled 2 and
3 that are delivered with the ACP215. To calibrate, follow the
instructions on the display (see Note 5).
6. After the Line Sensor Calibration check has been completed or
bypassed, the Bowl Seating Test will be performed to ensure
that the bowl is properly loaded. Upon the message “Is centrifuge cover locked?”, close the centrifuge lid and press YES. The
centrifuge will start running for 30 s. In the display the following message appears “Did bowl produce unusual noise.” Press
NO if the bowl turns smoothly without loud noise. If the bowl
produced loud noise, press YES. In this case discard the disposable and install a new disposable set.
530
Johan W. Lagerberg
second dilution, the diluted cells are transferred to the bowl where
the washing procedure in the bowl, using five washing steps with
0.9% NaCl/0.2% glucose solution, begins. Effluent exiting the
bowl will be collected in the waste bag. The line sensor monitors
the free hemoglobin level and cell spillage in the effluent. At the
completion of the washing procedure, the cells are washed once
with additive solution and finally resuspended in the additive solution. The deglycerolized RBCs are transferred from the washing
bowl into the blood product bag of the disposable set. The entire
deglycerolization procedure takes about 60–70 min.
3.2.1 Thawing the Unit
1. Remove the frozen unit from the freezer and from the
cardboard box.
2. Place the unit with the overwrap bag in a water bath maintained
at 40
C.
3. After 20 min, remove the overwrap and unfold the blood bag.
4. Monitor the temperature of the blood bag using a noncontact
thermometer. The temperature of the blood bag should be
between 25 and 30
C. Warm the unit if needed, taking care
not to wet the ports of the bag.
3.2.2 Deglycerolization
Procedure
1. Power-on the ACP215. The ACP215 automatically performs a
series of internal system checks.
2. Select the deglycerolization protocol.
3. Open the deglycerolization disposable set and inspect it for
visible defects.
4. Install the disposable set according to the manufacturer’s
instructions.
5. After loading the disposable set, the display posts the message
asking the operator if the Line Sensor Calibration Check is
needed. It is recommended to perform this check each day of
use. The line sensor is calibrated using the filters labeled 2 and
3 that are delivered with the ACP215. To calibrate, follow the
instructions on the display (see Note 5).
6. After the Line Sensor Calibration check has been completed or
bypassed, the Bowl Seating Test will be performed to ensure
that the bowl is properly loaded. Upon the message “Is centrifuge cover locked?”, close the centrifuge lid and press YES. The
centrifuge will start running for 30 s. In the display the following message appears “Did bowl produce unusual noise.” Press
NO if the bowl turns smoothly without loud noise. If the bowl
produced loud noise, press YES. In this case discard the disposable and install a new disposable set.
530
Johan W. Lagerberg