7. Styrofoam container.
8. Visotubes 10 mm (IMV 5561).
9. Cryo canes aluminum (Thermo Scientific Nalgene 50150001).
10. Cooper wire cutter (GB; GS-360; 0.80–2.6 mm).
2.2 Reagents
1. Sage Protein Supplement (SPS) (Sage).
2. Modified Human Tubal Fluid (HTF-HEPES) from Sage.
3. Vit Kit-Freeze (Irvine Scientific #90133DSOC).
4. Vit Kit-Thaw (Irvine Scientific # 90137DSOC).
2.3 Equipment
1. Dissecting stereomicroscope (Olympus SZX-12, Bausch
Lomb, or Leica) with warming stage.
2. Laminar flow hood (Origio).
3. Inverted microscope (Olympus 1X-71).
4. Infrared 1.48 μm diode laser (Hamilton Thorne – Zilos or
Lykos laser, Hamilton Thorne Research (Beverly, MA)).
Table 7
A comparison of retrospective data from the cryopreservation program (Fertility Centers of Illinois,
Chicago) of vitrified day 5 and day 6 blastocysts without AC (group A) and with AC (group B) using a
closed carrier system between 2007 and June 2019
Technique
Group A (no AC)
Group B (with AC)
Day 5
Day 6
Day 5
Day 6
Patient’s age (y)
35.8 Æ 5.0
36.1 Æ 4.9
35.2 Æ 4.5
36.4 Æ 4.4
Transfers
1423
924
4073
1807
Blastocysts warmed
2350
1611
5655
2600
Blastocysts survived
2329 (99.1)
1586 (98.4)
5642 (99.8)
2578 (99.2)
Blastocysts transferred
2268
1553
5600
2542
Blastocysts transferred (mean)
1.6
1.7
1.4
1.4
Implantations
892 (39.3)
*
435 (28.0)
*
2838 (50.7)
*
1052 (41.4)
*
Positive pregnancy/VET
826 (58.0)
**
419 (45.3)
**
2832 (69.5)
**
1115 (61.7)
**
Clinical pregnancy/VET
702 (49.3)
**
349 (37.8)
**
2415 (59.3)
**
903 (50.0)
**
Ongoing/delivered pregnancies
588 (41.3)
**
264 (28.6)
**
2106 (51.7)
**
749 (41.5)
**
*P < 0.01; **P < 0.001
VET vitrified embryo transfer. Values are numbers unless otherwise described, and percentages are indicated between
brackets
508
Juergen Liebermann
8. Visotubes 10 mm (IMV 5561).
9. Cryo canes aluminum (Thermo Scientific Nalgene 50150001).
10. Cooper wire cutter (GB; GS-360; 0.80–2.6 mm).
2.2 Reagents
1. Sage Protein Supplement (SPS) (Sage).
2. Modified Human Tubal Fluid (HTF-HEPES) from Sage.
3. Vit Kit-Freeze (Irvine Scientific #90133DSOC).
4. Vit Kit-Thaw (Irvine Scientific # 90137DSOC).
2.3 Equipment
1. Dissecting stereomicroscope (Olympus SZX-12, Bausch
Lomb, or Leica) with warming stage.
2. Laminar flow hood (Origio).
3. Inverted microscope (Olympus 1X-71).
4. Infrared 1.48 μm diode laser (Hamilton Thorne – Zilos or
Lykos laser, Hamilton Thorne Research (Beverly, MA)).
Table 7
A comparison of retrospective data from the cryopreservation program (Fertility Centers of Illinois,
Chicago) of vitrified day 5 and day 6 blastocysts without AC (group A) and with AC (group B) using a
closed carrier system between 2007 and June 2019
Technique
Group A (no AC)
Group B (with AC)
Day 5
Day 6
Day 5
Day 6
Patient’s age (y)
35.8 Æ 5.0
36.1 Æ 4.9
35.2 Æ 4.5
36.4 Æ 4.4
Transfers
1423
924
4073
1807
Blastocysts warmed
2350
1611
5655
2600
Blastocysts survived
2329 (99.1)
1586 (98.4)
5642 (99.8)
2578 (99.2)
Blastocysts transferred
2268
1553
5600
2542
Blastocysts transferred (mean)
1.6
1.7
1.4
1.4
Implantations
892 (39.3)
*
435 (28.0)
*
2838 (50.7)
*
1052 (41.4)
*
Positive pregnancy/VET
826 (58.0)
**
419 (45.3)
**
2832 (69.5)
**
1115 (61.7)
**
Clinical pregnancy/VET
702 (49.3)
**
349 (37.8)
**
2415 (59.3)
**
903 (50.0)
**
Ongoing/delivered pregnancies
588 (41.3)
**
264 (28.6)
**
2106 (51.7)
**
749 (41.5)
**
*P < 0.01; **P < 0.001
VET vitrified embryo transfer. Values are numbers unless otherwise described, and percentages are indicated between
brackets
508
Juergen Liebermann