7. If Castroviejo needle driver is not available, suturing can alternatively be performed with watchmaker or other fine forceps.
8. Working under the laminar flow hood for an extended time
period may dry the cornea of the technician. Thus, it is important to protect the personal from dry eye.
9. This chapter largely describes OT cryopreservation and orthotropic transplantation for laboratory mice and rats with the
intention of producing offspring. However, the cryopreservation procedures described here can be used to perform heterotopic transplantation such as under the renal capsule (see
Fig. 5a–c), subcutaneous (see Fig. 6a–c), intramuscular, or
other areas with some modifications in the standard surgical
producers to access to desired graft site [32–37].
10. Animals maybe initially stressed if they are not properly handled. To avoid this, it is important to practice the procedure
with few times before handling the experimental animals. It is
also important not to touch deep into the vagina and stimulate
the cervix since this would induce pseudopregnancy in the
female.
Fig. 5 Frozen-thaw rat ovarian tissue xenografting beneath the renal capsule of athymic nude mice following
bilateral ovariectomy (a), gross (b) and histologic cross section of ovarian tissue graft containing preovulatory
follicles (c)
Fig. 6 Subcutaneous frozen-thaw rat ovarian tissue autografting (arrows) following bilateral ovariectomy (a),
gross (b) and histologic cross section of ovarian tissue graft containing preovulatory follicles (c)
Rodent Ovarian Tissue Cryopreservation
481
8. Working under the laminar flow hood for an extended time
period may dry the cornea of the technician. Thus, it is important to protect the personal from dry eye.
9. This chapter largely describes OT cryopreservation and orthotropic transplantation for laboratory mice and rats with the
intention of producing offspring. However, the cryopreservation procedures described here can be used to perform heterotopic transplantation such as under the renal capsule (see
Fig. 5a–c), subcutaneous (see Fig. 6a–c), intramuscular, or
other areas with some modifications in the standard surgical
producers to access to desired graft site [32–37].
10. Animals maybe initially stressed if they are not properly handled. To avoid this, it is important to practice the procedure
with few times before handling the experimental animals. It is
also important not to touch deep into the vagina and stimulate
the cervix since this would induce pseudopregnancy in the
female.
Fig. 5 Frozen-thaw rat ovarian tissue xenografting beneath the renal capsule of athymic nude mice following
bilateral ovariectomy (a), gross (b) and histologic cross section of ovarian tissue graft containing preovulatory
follicles (c)
Fig. 6 Subcutaneous frozen-thaw rat ovarian tissue autografting (arrows) following bilateral ovariectomy (a),
gross (b) and histologic cross section of ovarian tissue graft containing preovulatory follicles (c)
Rodent Ovarian Tissue Cryopreservation
481