As you can see, all of the relevant information is available
for ordering genotyping primers, such as sequence and melting
temperatures. Here, the Tm’s are similar enough for all three
primers being used in a single duplex PCR reaction.
3.13 Conclusions
In summary, it is clear that there is an enormous volume of data at
the disposal of Arabidopsis researchers, and by accessing it with the
tools described in this lab and others, insights can be garnered that
will help with elucidating the role of a gene or set of genes in
question. As a final note, the International Arabidopsis Informatics
Consortium [83] has worked with developers and researchers to
launch an Arabidopsis Information Portal, Araport.org, which
brings together the data described for the tools above and from
elsewhere into a seamless interface. Data is constantly being added
to this database and to many of the databases described in this
chapter, so it is worthwhile to check them all frequently.
4 Notes
1. Available at http://bar.utoronto.ca/~nprovart/Genes_up_in_
Lec1OX_w_decimal.txt
2. Not the case in Safari!
Fig. 30 T-DNA primer design for mutant genotyping
Arabidopsis Bioinformatics
83