2. There is one SALK line of interest in the first exon of ABI3,
SALK_138922. Generally speaking, when selecting insertional
mutants, it is always best to select gene disruptions that are in
the first exon (see Fig. 29).
3. Click on “SALK_138922.” You will be taken to a page with
more detailed information about SALK_138922. In this case,
the mutant is described as a knockout for ABI3, which is the
most ideal case. To order this mutant, click on “Order from
ABRC.”
4. You will be taken to TAIR where additional information about
this T-DNA line will be displayed. Some information worth
noting about any mutant line are the name of parental line,
ploidy number or whether the mutation is homozygous. To
order, click on “Order from ABRC” (see Note 27).
Fig. 29 Screenshot of T-DNA Express genome browser at the ABI3 locus. Genes and T-DNA insertions are
displayed
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