INDEX
A
Acquire X ........................... 414, 415, 417–419, 421–423
Affinity purification .............................................. 432, 436
Agnostic sampling ................................................ 256, 270
Agrobacterium tumefaciens................................. 4, 15, 16,
80, 126, 136, 148, 307, 327, 430
Amyloplasts........................................................... 167, 177
Anticlinal walls............................................. 354, 356, 366
Atomic force microscopy (AFM) ....................... 350–355,
357, 359–361, 363, 367, 368
B
Base editors........................................................... 124, 143
Bimolecular fluorescent complementation
(BiFC) ................................................................ 324
BioID ..........................................425, 426, 432, 433, 438
Biotinylation ................................................ 425, 426, 438
C
Calli ......................................................167, 173–178, 184
Cell suspension cultures.............................................8, 17,
160, 167–185
Cell walls........................................ 8, 168, 256, 259, 266,
337, 349–368, 373
Chloroplasts....................................... 154, 167, 168, 176,
258, 267, 274, 276, 332, 341
Chromatin mobility ...................................................... 214
Coexpression ........................................41, 42, 44, 45, 48,
70, 72, 74, 75, 85
Colocalization analysis .................................................. 163
Computational analysis ............................... 225, 247, 295
Confocal ............................................ 148, 151, 154, 158,
159, 161–163, 176, 215, 217, 221, 295–301,
318, 324, 328, 332, 350
Confocal imaging ........................................ 161, 163, 164
CpFP .............................................................................. 307
CRISPR/Cas9............................................. 122, 124, 131
Cross-linking .......................................453, 454, 459, 460
Culture medium................................ 158, 159, 161, 167,
169–172, 174, 176, 177, 179, 181–184, 259, 266
E
eFP, bioinformatics .................................... 27, 36–40, 46,
53, 65–66, 71, 75, 76, 84
Elasticity....................................................... 350, 353, 363
Elastic modulus ............................................352, 363–366
Embryogenesis ................................................................ 13
Embryo sacs.........................................372, 374, 382–385
Environmental conditions ......................... 3, 4, 8, 10–12,
14, 19, 20, 22, 416
Environmental variation ...................................... 102, 108
Epidermis.............................................116, 151, 216, 331
F
Fertilization ........................... 8, 371, 374, 382, 384, 388
Field experiments ....................................................93–109
Flow cytometry ................................................... 255, 256,
258–263, 265–269, 272
Fluorescent markers .................................... 150, 151, 154
Fluorescent proteins................................... 148, 151, 159,
164, 214, 216, 256, 258, 259, 261, 262, 265,
284, 295, 300, 305–307, 309, 324
Fo ¨rster resonance energy transfer
(FRET)............................306, 307, 309, 310, 323
Freeze-substitution .............................................. 337–347
Functional classification ..................................... 55–57, 59
Functional genomics......................................15, 121, 188
G
Garden experiment ................................................ 94, 101
Gas-chromatography mass-spectrometry
(GC-MS)......................... 394–396, 398, 404, 405
Gene expression ..................................... 8, 32, 37, 40–42,
46, 52, 53, 55, 74, 75, 100, 122, 153, 225, 250,
256, 268, 285, 286, 295, 310, 323
Genetically encoded fluorescent
biosensors ................................................. 303–319
Genetic crosses ................................................... 3–22, 372
Genetics ............................................. 4, 9, 11, 15, 18, 26,
30, 70, 72, 74, 78, 93–95, 98, 99, 101, 106, 108,
109, 121, 122, 140, 148, 157, 187, 188, 194,
195, 310, 397–399, 402, 403, 405–408, 415, 416
Genome-wide association
(GWA).......................................... 94, 99, 109, 399
Genome wide association mapping.............................. 394
Genome wide association study
(GWAS)..................................................... 188, 398
Genotype-to-phenotype association .................. 187–188,
397
Jose J. Sanchez-Serrano and Julio Salinas (eds.), Arabidopsis Protocols, Methods in Molecular Biology, vol. 2200,
https://doi.org/10.1007/978-1-0716-0880-7, © Springer Science+Business Media, LLC, part of Springer Nature 2021
463
A
Acquire X ........................... 414, 415, 417–419, 421–423
Affinity purification .............................................. 432, 436
Agnostic sampling ................................................ 256, 270
Agrobacterium tumefaciens................................. 4, 15, 16,
80, 126, 136, 148, 307, 327, 430
Amyloplasts........................................................... 167, 177
Anticlinal walls............................................. 354, 356, 366
Atomic force microscopy (AFM) ....................... 350–355,
357, 359–361, 363, 367, 368
B
Base editors........................................................... 124, 143
Bimolecular fluorescent complementation
(BiFC) ................................................................ 324
BioID ..........................................425, 426, 432, 433, 438
Biotinylation ................................................ 425, 426, 438
C
Calli ......................................................167, 173–178, 184
Cell suspension cultures.............................................8, 17,
160, 167–185
Cell walls........................................ 8, 168, 256, 259, 266,
337, 349–368, 373
Chloroplasts....................................... 154, 167, 168, 176,
258, 267, 274, 276, 332, 341
Chromatin mobility ...................................................... 214
Coexpression ........................................41, 42, 44, 45, 48,
70, 72, 74, 75, 85
Colocalization analysis .................................................. 163
Computational analysis ............................... 225, 247, 295
Confocal ............................................ 148, 151, 154, 158,
159, 161–163, 176, 215, 217, 221, 295–301,
318, 324, 328, 332, 350
Confocal imaging ........................................ 161, 163, 164
CpFP .............................................................................. 307
CRISPR/Cas9............................................. 122, 124, 131
Cross-linking .......................................453, 454, 459, 460
Culture medium................................ 158, 159, 161, 167,
169–172, 174, 176, 177, 179, 181–184, 259, 266
E
eFP, bioinformatics .................................... 27, 36–40, 46,
53, 65–66, 71, 75, 76, 84
Elasticity....................................................... 350, 353, 363
Elastic modulus ............................................352, 363–366
Embryogenesis ................................................................ 13
Embryo sacs.........................................372, 374, 382–385
Environmental conditions ......................... 3, 4, 8, 10–12,
14, 19, 20, 22, 416
Environmental variation ...................................... 102, 108
Epidermis.............................................116, 151, 216, 331
F
Fertilization ........................... 8, 371, 374, 382, 384, 388
Field experiments ....................................................93–109
Flow cytometry ................................................... 255, 256,
258–263, 265–269, 272
Fluorescent markers .................................... 150, 151, 154
Fluorescent proteins................................... 148, 151, 159,
164, 214, 216, 256, 258, 259, 261, 262, 265,
284, 295, 300, 305–307, 309, 324
Fo ¨rster resonance energy transfer
(FRET)............................306, 307, 309, 310, 323
Freeze-substitution .............................................. 337–347
Functional classification ..................................... 55–57, 59
Functional genomics......................................15, 121, 188
G
Garden experiment ................................................ 94, 101
Gas-chromatography mass-spectrometry
(GC-MS)......................... 394–396, 398, 404, 405
Gene expression ..................................... 8, 32, 37, 40–42,
46, 52, 53, 55, 74, 75, 100, 122, 153, 225, 250,
256, 268, 285, 286, 295, 310, 323
Genetically encoded fluorescent
biosensors ................................................. 303–319
Genetic crosses ................................................... 3–22, 372
Genetics ............................................. 4, 9, 11, 15, 18, 26,
30, 70, 72, 74, 78, 93–95, 98, 99, 101, 106, 108,
109, 121, 122, 140, 148, 157, 187, 188, 194,
195, 310, 397–399, 402, 403, 405–408, 415, 416
Genome-wide association
(GWA).......................................... 94, 99, 109, 399
Genome wide association mapping.............................. 394
Genome wide association study
(GWAS)..................................................... 188, 398
Genotype-to-phenotype association .................. 187–188,
397
Jose J. Sanchez-Serrano and Julio Salinas (eds.), Arabidopsis Protocols, Methods in Molecular Biology, vol. 2200,
https://doi.org/10.1007/978-1-0716-0880-7, © Springer Science+Business Media, LLC, part of Springer Nature 2021
463