RootChip
technology.
New
Phytol
202:198–208
19. Fehr M, Lalonde S, Lager I, Wolff MW, Frommer WB (2003) In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7
cells by fluorescent nanosensors. J Biol Chem
278:19127–19133
20. Lager I, Looger LL, Hilpert M, Lalonde S,
Frommer WB (2006) Conversion of a putative
agrobacterium sugar-binding protein into a
FRET sensor with high selectivity for sucrose.
J Biol Chem 281:30875–30883
21. Chen L-Q, Hou B-H, Lalonde S, Takanaga H,
Hartung ML, Qu X-Q et al (2010) Sugar
transporters for intercellular exchange and
nutrition of pathogens. Nature 468:527–532
22. Chen L-Q, Qu X-Q, Hou B-H, Sosso D,
Osorio S, Fernie AR et al (2012) Sucrose efflux
mediated by SWEET proteins as a key step for
phloem transport. Science 335:207–211
23. Deuschle K, Okumoto S, Fehr M, Looger LL,
Kozhukh L, Frommer WB (2005) Construction and optimization of a family of genetically
encoded metabolite sensors by semirational
protein
engineering.
Protein
Sci
14:2304–2314
24. Okumoto S, Looger LL, Micheva KD, Reimer
RJ, Smith SJ, Frommer WB (2005) Detection
of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Proc Natl Acad Sci U S A
102:8740–8745
25. Imamura H, Huynh KP, Togawa H, Saito K,
Iino R, Kato-yamada Y (2009) Visualization of
ATP levels inside single living cells with fluorescence resonance energy transfer-based. Proc
Natl Acad Sci U S A 106:15651–15656
26. Hatsugai N, Perez Koldenkova V, Imamura H,
Noji H, Nagai T (2012) Changes in cytosolic
ATP levels and intracellular morphology during bacteria-induced hypersensitive cell death
as revealed by real-time fluorescence microscopy
imaging.
Plant
Cell
Physiol
53:1768–1775
27. De Col V, Fuchs P, Nietzel T, Els€ asser M, Voon
CP, Candeo A et al (2017) ATP sensing in
living plant cells reveals tissue gradients and
stress dynamics of energy physiology. elife 6:
e26770
28. Waadt R, Hitomi K, Nishimura N, Hitomi C,
Adams SR, Getzoff ED, Schroeder JI (2014)
FRET-based reporters for the direct visualization of abscisic acid concentration changes and
distribution in Arabidopsis. elife 3:e01739
29. Jones AM, JAH D, Manojkumar SN,
Lanquar V, Grossmann G, Frommer WB
(2014) Abscisic acid dynamics in roots
detected with genetically encoded FRET sensors. elife 3:e01741
30. Rizza A, Walia A, Lanquar V, Frommer WB,
Jones AM (2017) In vivo gibberellin gradients
visualized in rapidly elongating tissues. Nat
Plants 3:803–813
31. Kneen M, Farinas J, Li Y, Verkman AS (1998)
Green fluorescent protein as a noninvasive
intracellular pH indicator. Biophys J
74:1591–1599
32. Jayaraman S, Haggie P, Wachter RM, Remington SJ, Verkman AS (2000) Mechanism and
cellular applications of a green fluorescent
protein-based halide sensor. J Biol Chem
275:6047–6050
33. Shen J, Zeng Y, Zhuang X, Sun L, Yao X,
Pimpl P, Jiang L (2013) Organelle pH in the
Arabidopsis endomembrane system. Mol Plant
6:1419–1437
34. Martiniere A, Bassil E, Jublanc E, Alcon C,
Reguera M, Sentenac H et al (2013) In vivo
intracellular pH measurements in tobacco and
Arabidopsis reveal an unexpected pH gradient
in the endomembrane system. Plant Cell
25:4028–4043
35. Yang H, Pu X, Wang L, Liu L, Theg SM
(2017) A new fluorescence-based method to
monitor the pH in the thylakoid lumen using
GFP variants. Biochem Biophys Res Commun
486:1–5
36. Monshausen GB, Bibikova TN, Weisenseel
MH, Gilroy S (2009) Ca
2+ regulates reactive
oxygen species production and pH during
mechanosensing in Arabidopsis roots. Plant
Cell 21:2341–2356
37. Gjetting KSK, Ytting CK, Schulz A, Fuglsang
AT (2012) Live imaging of intra-and extracellular pH in plants using pHusion, a novel
genetically encoded biosensor. J Exp Bot
63:3207–3218
38. Hanson GT, Aggeler R, Oglesbee D,
Cannon M, Capaldi RA, Tsien RY, Remington
SJ (2004) Investigating mitochondrial redox
potential with redox-sensitive green fluorescent protein indicators. J Biol Chem
279:13044–13053
39. Meyer AJ, Brach T, Marty L, Kreye S,
Rouhier N, Jacquot JP, Hell R (2007) Redoxsensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the
cellular glutathione redox buffer. Plant J
52:973–986
40. Schwarzl€ ander M, Fricker MD, Sweetlove LJ
(2009) Monitoring the in vivo redox state of
plant mitochondria: effect of respiratory inhibitors, abiotic stress and assessment of recovery
320
Akira Yoshinari et al.
technology.
New
Phytol
202:198–208
19. Fehr M, Lalonde S, Lager I, Wolff MW, Frommer WB (2003) In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7
cells by fluorescent nanosensors. J Biol Chem
278:19127–19133
20. Lager I, Looger LL, Hilpert M, Lalonde S,
Frommer WB (2006) Conversion of a putative
agrobacterium sugar-binding protein into a
FRET sensor with high selectivity for sucrose.
J Biol Chem 281:30875–30883
21. Chen L-Q, Hou B-H, Lalonde S, Takanaga H,
Hartung ML, Qu X-Q et al (2010) Sugar
transporters for intercellular exchange and
nutrition of pathogens. Nature 468:527–532
22. Chen L-Q, Qu X-Q, Hou B-H, Sosso D,
Osorio S, Fernie AR et al (2012) Sucrose efflux
mediated by SWEET proteins as a key step for
phloem transport. Science 335:207–211
23. Deuschle K, Okumoto S, Fehr M, Looger LL,
Kozhukh L, Frommer WB (2005) Construction and optimization of a family of genetically
encoded metabolite sensors by semirational
protein
engineering.
Protein
Sci
14:2304–2314
24. Okumoto S, Looger LL, Micheva KD, Reimer
RJ, Smith SJ, Frommer WB (2005) Detection
of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Proc Natl Acad Sci U S A
102:8740–8745
25. Imamura H, Huynh KP, Togawa H, Saito K,
Iino R, Kato-yamada Y (2009) Visualization of
ATP levels inside single living cells with fluorescence resonance energy transfer-based. Proc
Natl Acad Sci U S A 106:15651–15656
26. Hatsugai N, Perez Koldenkova V, Imamura H,
Noji H, Nagai T (2012) Changes in cytosolic
ATP levels and intracellular morphology during bacteria-induced hypersensitive cell death
as revealed by real-time fluorescence microscopy
imaging.
Plant
Cell
Physiol
53:1768–1775
27. De Col V, Fuchs P, Nietzel T, Els€ asser M, Voon
CP, Candeo A et al (2017) ATP sensing in
living plant cells reveals tissue gradients and
stress dynamics of energy physiology. elife 6:
e26770
28. Waadt R, Hitomi K, Nishimura N, Hitomi C,
Adams SR, Getzoff ED, Schroeder JI (2014)
FRET-based reporters for the direct visualization of abscisic acid concentration changes and
distribution in Arabidopsis. elife 3:e01739
29. Jones AM, JAH D, Manojkumar SN,
Lanquar V, Grossmann G, Frommer WB
(2014) Abscisic acid dynamics in roots
detected with genetically encoded FRET sensors. elife 3:e01741
30. Rizza A, Walia A, Lanquar V, Frommer WB,
Jones AM (2017) In vivo gibberellin gradients
visualized in rapidly elongating tissues. Nat
Plants 3:803–813
31. Kneen M, Farinas J, Li Y, Verkman AS (1998)
Green fluorescent protein as a noninvasive
intracellular pH indicator. Biophys J
74:1591–1599
32. Jayaraman S, Haggie P, Wachter RM, Remington SJ, Verkman AS (2000) Mechanism and
cellular applications of a green fluorescent
protein-based halide sensor. J Biol Chem
275:6047–6050
33. Shen J, Zeng Y, Zhuang X, Sun L, Yao X,
Pimpl P, Jiang L (2013) Organelle pH in the
Arabidopsis endomembrane system. Mol Plant
6:1419–1437
34. Martiniere A, Bassil E, Jublanc E, Alcon C,
Reguera M, Sentenac H et al (2013) In vivo
intracellular pH measurements in tobacco and
Arabidopsis reveal an unexpected pH gradient
in the endomembrane system. Plant Cell
25:4028–4043
35. Yang H, Pu X, Wang L, Liu L, Theg SM
(2017) A new fluorescence-based method to
monitor the pH in the thylakoid lumen using
GFP variants. Biochem Biophys Res Commun
486:1–5
36. Monshausen GB, Bibikova TN, Weisenseel
MH, Gilroy S (2009) Ca
2+ regulates reactive
oxygen species production and pH during
mechanosensing in Arabidopsis roots. Plant
Cell 21:2341–2356
37. Gjetting KSK, Ytting CK, Schulz A, Fuglsang
AT (2012) Live imaging of intra-and extracellular pH in plants using pHusion, a novel
genetically encoded biosensor. J Exp Bot
63:3207–3218
38. Hanson GT, Aggeler R, Oglesbee D,
Cannon M, Capaldi RA, Tsien RY, Remington
SJ (2004) Investigating mitochondrial redox
potential with redox-sensitive green fluorescent protein indicators. J Biol Chem
279:13044–13053
39. Meyer AJ, Brach T, Marty L, Kreye S,
Rouhier N, Jacquot JP, Hell R (2007) Redoxsensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the
cellular glutathione redox buffer. Plant J
52:973–986
40. Schwarzl€ ander M, Fricker MD, Sweetlove LJ
(2009) Monitoring the in vivo redox state of
plant mitochondria: effect of respiratory inhibitors, abiotic stress and assessment of recovery
320
Akira Yoshinari et al.