Acknowledgements
We thank Mr. Koji Matsushita (Nagoya University) for designing
the perfusion chamber holder. This research was supported by the
Human Frontier Science Program to M.N., JSPS Grant-in-Aid for
Young Scientists (19K16164) to A.Y., and JSPS KAKENHI Grant
(18KK0195) to M.N. and A.Y., and ITbM is supported by World
Premier International Research Center Initiative (WPI), Japan.
References
1. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary
structure of the Aequorea victoria greenfluorescent protein (Bioluminescence; Cnidaria; aequorin; energy transfer; chromophore;
cloning). Biochem Mol Biol Mayo Found
111:284–2065
2. Gross LA, Baird GS, Hoffman RC, Baldridge
KK, Tsien RY (2000) The structure of the
chromophore within DsRed, a red fluorescent
protein from coral. Proc Natl Acad Sci U S A
97:11990–11995
3. Ormo ¨ M, Cubitt AB, Kallio K, Gross LA, Tsien
RY, Remington SJ (1996) Crystal structure of
the Aequorea victoria green fluorescent protein. Science 273:1392–1395
4. Ulmasov T, Murfett J, Hagen G, Guilfoyle T
(1997) Creation of a highly active synthetic
AuxRE. Plant Cell 9:1963–1971
5. Brunoud G, Wells DM, Oliva M, Larrieu A,
Mirabet V, Burrow AH et al (2012) A novel
sensor to map auxin response and distribution
at high spatio-temporal resolution. Nature
482:103–106
6. Silverstone AL (2001) Repressing a repressor:
gibberellin-induced rapid reduction of the
RGA protein in Arabidopsis. Plant Cell
13:1555–1566
7. Silverstone AL, Tseng T-S, Swain SM, Dill A,
Jeong SY, Olszewski NE, Sun T-P (2006)
Functional analysis of SPINDLY in gibberellin
signaling in Arabidopsis. Plant Physiol
143:987–1000
8. Larrieu A, Champion A, Legrand J, Lavenus J,
Mast D, Brunoud G et al (2015) A fluorescent
hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nat Commun
6:6043
9. Samodelov SL, Beyer HM, Guo X, Augustin M,
Jia KP, Baz L et al (2016) Strigoquant: a genetically encoded biosensor for quantifying Strigolactone activity and specificity. Sci Adv 2:
e1601266
10. Tanaka M, Takano J, Chiba Y, Lombardo F,
Ogasawara Y, Onouchi H et al (2011) Borondependent degradation of NIP5;1 mRNA for
acclimation to excess boron conditions in Arabidopsis. Plant Cell 23:3547–3559
11. Tanaka M, Sotta N, Yamazumi Y, Yamashita Y,
Miwa K, Murota K et al (2016) The minimum
open Reading frame, AUG-stop, induces
boron-dependent ribosome stalling and
mRNA degradation. Plant Cell 28:2830–2849
12. Fukuda M, Wakuta S, Kamiyo J, Fujiwara T,
Takano J (2018) Establishment of genetically
encoded biosensors for cytosolic boric acid in
plant cells. Plant J 95:763–774
13. Miyawaki A, Llopis J, Heim R, McCaffery JM,
Adams JA, Ikura M, Tsien RY (1997) Fluorescent indicators for Ca
2+ based on green fluorescent proteins and calmodulin. Nature
388:882–887
14. Allen GJ, Kwak JM, Chu SP, Llopis J, Tsien
RY, Harper JF, Schroeder JI (1999) Cameleon
calcium indicator reports cytoplasmic calcium
dynamics in Arabidopsis guard cells. Plant J
19:735–747
15. Allen GJ, Chu SP, Harrington CL,
Schumacher K, Hoffmann T, Tang YY et al
(2001) A defined range of guard cell calcium
oscillation parameters encodes stomatal movements. Nature 411:1053–1057
16. Mukherjee P, Banerjee S, Wheeler A, Ratliff
LA, Irigoyen S, Garcia LR et al (2015) Live
imaging of inorganic phosphate in plants with
cellular and subcellular resolution. Plant
Physiol 167:628–638
17. Chabosseau P, Tuncay E, Meur G, Bellomo
EA, Hessels A, Hughes S et al (2014) Mitochondrial and ER-targeted eCALWY probes
reveal high levels of free Zn
2+
. ACS Chem
Biol 9:2111–2120
18. Lanquar V, Grossmann G, Vinkenborg JL,
Merkx M, Thomine S, Frommer WB (2014)
Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and
The Use of Genetically Encoded Fluorescent Biosensors
319
We thank Mr. Koji Matsushita (Nagoya University) for designing
the perfusion chamber holder. This research was supported by the
Human Frontier Science Program to M.N., JSPS Grant-in-Aid for
Young Scientists (19K16164) to A.Y., and JSPS KAKENHI Grant
(18KK0195) to M.N. and A.Y., and ITbM is supported by World
Premier International Research Center Initiative (WPI), Japan.
References
1. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary
structure of the Aequorea victoria greenfluorescent protein (Bioluminescence; Cnidaria; aequorin; energy transfer; chromophore;
cloning). Biochem Mol Biol Mayo Found
111:284–2065
2. Gross LA, Baird GS, Hoffman RC, Baldridge
KK, Tsien RY (2000) The structure of the
chromophore within DsRed, a red fluorescent
protein from coral. Proc Natl Acad Sci U S A
97:11990–11995
3. Ormo ¨ M, Cubitt AB, Kallio K, Gross LA, Tsien
RY, Remington SJ (1996) Crystal structure of
the Aequorea victoria green fluorescent protein. Science 273:1392–1395
4. Ulmasov T, Murfett J, Hagen G, Guilfoyle T
(1997) Creation of a highly active synthetic
AuxRE. Plant Cell 9:1963–1971
5. Brunoud G, Wells DM, Oliva M, Larrieu A,
Mirabet V, Burrow AH et al (2012) A novel
sensor to map auxin response and distribution
at high spatio-temporal resolution. Nature
482:103–106
6. Silverstone AL (2001) Repressing a repressor:
gibberellin-induced rapid reduction of the
RGA protein in Arabidopsis. Plant Cell
13:1555–1566
7. Silverstone AL, Tseng T-S, Swain SM, Dill A,
Jeong SY, Olszewski NE, Sun T-P (2006)
Functional analysis of SPINDLY in gibberellin
signaling in Arabidopsis. Plant Physiol
143:987–1000
8. Larrieu A, Champion A, Legrand J, Lavenus J,
Mast D, Brunoud G et al (2015) A fluorescent
hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nat Commun
6:6043
9. Samodelov SL, Beyer HM, Guo X, Augustin M,
Jia KP, Baz L et al (2016) Strigoquant: a genetically encoded biosensor for quantifying Strigolactone activity and specificity. Sci Adv 2:
e1601266
10. Tanaka M, Takano J, Chiba Y, Lombardo F,
Ogasawara Y, Onouchi H et al (2011) Borondependent degradation of NIP5;1 mRNA for
acclimation to excess boron conditions in Arabidopsis. Plant Cell 23:3547–3559
11. Tanaka M, Sotta N, Yamazumi Y, Yamashita Y,
Miwa K, Murota K et al (2016) The minimum
open Reading frame, AUG-stop, induces
boron-dependent ribosome stalling and
mRNA degradation. Plant Cell 28:2830–2849
12. Fukuda M, Wakuta S, Kamiyo J, Fujiwara T,
Takano J (2018) Establishment of genetically
encoded biosensors for cytosolic boric acid in
plant cells. Plant J 95:763–774
13. Miyawaki A, Llopis J, Heim R, McCaffery JM,
Adams JA, Ikura M, Tsien RY (1997) Fluorescent indicators for Ca
2+ based on green fluorescent proteins and calmodulin. Nature
388:882–887
14. Allen GJ, Kwak JM, Chu SP, Llopis J, Tsien
RY, Harper JF, Schroeder JI (1999) Cameleon
calcium indicator reports cytoplasmic calcium
dynamics in Arabidopsis guard cells. Plant J
19:735–747
15. Allen GJ, Chu SP, Harrington CL,
Schumacher K, Hoffmann T, Tang YY et al
(2001) A defined range of guard cell calcium
oscillation parameters encodes stomatal movements. Nature 411:1053–1057
16. Mukherjee P, Banerjee S, Wheeler A, Ratliff
LA, Irigoyen S, Garcia LR et al (2015) Live
imaging of inorganic phosphate in plants with
cellular and subcellular resolution. Plant
Physiol 167:628–638
17. Chabosseau P, Tuncay E, Meur G, Bellomo
EA, Hessels A, Hughes S et al (2014) Mitochondrial and ER-targeted eCALWY probes
reveal high levels of free Zn
2+
. ACS Chem
Biol 9:2111–2120
18. Lanquar V, Grossmann G, Vinkenborg JL,
Merkx M, Thomine S, Frommer WB (2014)
Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and
The Use of Genetically Encoded Fluorescent Biosensors
319