3.3 Time-Lapse
Imaging of Leaves
and Vegetative
Meristems
shot of the first pair of leaves and meristem but also possible to
perform time-lapse imaging to follow development over time. This
section aims to provide some advice and tips for such time-lapse
imaging (also see Notes 2–4).
1. Since seedlings are still alive after dissection, it is important
during a time-lapse experiment to take into account vertical
growth. To compensate for this, adjust the starting point for
each z-stack appropriately for each time or in the case of an
unsupervised acquisition, set the starting point of the z-stack
Fig. 3 Time lapse of the first pair of leaves. (a) Time-lapse images of the first pair of leaves in a 3 DAS
seedling. Nuclei are marked with mCherry nuclear-localized fluorescent protein (from [8]). Snapshots of the
same seedling imaged every hour. (b) The seedling used for the time-lapse experiment 1 day later, confirming
that it was still alive and continuing to grow
300
Monica Pia Caggiano et al.
Imaging of Leaves
and Vegetative
Meristems
shot of the first pair of leaves and meristem but also possible to
perform time-lapse imaging to follow development over time. This
section aims to provide some advice and tips for such time-lapse
imaging (also see Notes 2–4).
1. Since seedlings are still alive after dissection, it is important
during a time-lapse experiment to take into account vertical
growth. To compensate for this, adjust the starting point for
each z-stack appropriately for each time or in the case of an
unsupervised acquisition, set the starting point of the z-stack
Fig. 3 Time lapse of the first pair of leaves. (a) Time-lapse images of the first pair of leaves in a 3 DAS
seedling. Nuclei are marked with mCherry nuclear-localized fluorescent protein (from [8]). Snapshots of the
same seedling imaged every hour. (b) The seedling used for the time-lapse experiment 1 day later, confirming
that it was still alive and continuing to grow
300
Monica Pia Caggiano et al.