microscopy of multiple organelles in Arabidopsis seedlings. Plant Methods 4:9.
https://doi.org/10.1186/1746-4811-4-9
123. Hirakawa T, Matsunaga S (2016) Threedimensional, live-cell imaging of chromatin
dynamics in plant nuclei using chromatin tagging
systems.
In:
Murata
M
(ed) Chromosome and genomic engineering
in plants, Methods in molecular biology, vol
1469. Humana Press, New York, pp 189–195
124. Ford K, McDonald D, Mali P (2019) Functional genomics via CRISPR-Cas. J Mol Biol
431:48–65. Special Issue: SI
125. Galbraith DW, Mauch TJ, Shields BA (1981)
Analysis of the initial stages of plant protoplast
development using 33258 Hoechst: reactivation of the cell cycle. Physiol Plant
51:380–386
126. Galbraith DW (1981) Microfluorometric
quantitation of cellulose biosynthesis by
plant protoplasts using Calcofluor White.
Physiol Plant 53:111–116
127. Galbraith DW, Shields BA (1982) The effects
of inhibitors of cell wall synthesis on tobacco
protoplast development. Physiol Plant
55:25–30
128. Galbraith DW, Lucretti S (2000) Large particle sorting. In: Radbruch A (ed) Flow cytometry and cell sorting, 2nd edn. Springer,
Berlin, pp 293–317
129. Harkins KR, Galbraith DW (1987) Factors
governing the flow cytometric analysis and
sorting of large biological particles. Cytometry 8:60–71
130. Galbraith DW (2009) Simultaneous flow
cytometric quantification of plant nuclear
DNA contents over the full range of described
angiosperm 2C values. Cytometry A
75A:692–698
131. Shapiro H (2003) Practical flow cytometry,
4th edn. John Wiley, Hoboken
132. Shendure J, Balasubramanian S, Church GM,
Gilbert W, Rogers J, Schloss JA, Waterston
RH (2017) DNA sequencing at 40: past,
present and future. Nature 550:345–353
133. Zheng GXY et al (2017) Massively parallel
digital transcriptional profiling of single cells.
Nat Commun 8:Article Number: 14049.
https://doi.org/10.1038/ncomms14049
134. Rotman N, Durbarry A, Wardle A, Yang WC,
Chaboud A, Faure JE, Berger F, Twell D
(2005) A novel class of MYB factors controls
sperm-cell formation in plants. Curr Biol
15:244–248
135. Borges F, Rui Gardner R, Lopes T, Calarco
JP, Boavida LC, Slotkin RK, Martienssen RA,
Becker JD (2012) FACS-based purification of
Arabidopsis microspores, sperm cells and vegetative nuclei. Plant Methods 8:44
136. Ingouff M, Hamamura Y, Gourgues M,
Higashiyama T, Berger F (2007) Distinct
dynamics of HISTONE3 variants between
the two fertilization products in plants. Curr
Biol 12:1032–1037
137. Misra CS, Santos MR, Rafael-Fernandes M,
Martins NP, Monteiro M, Becker JD (2019)
Transcriptomics of Arabidopsis sperm cells at
single-cell resolution. Plant Reprod 32:29–38
138. Yelina NE, Ziolkowski PA, Miller N, Zhao
XH, Kelly KA, Munoz DF, Mann DJ, Copenhaver GP, Henderson IR (2013) Highthroughput analysis of meiotic crossover frequency and interference via flow cytometry of
fluorescent pollen in Arabidopsis thaliana.
Nat Protoc 8:2119–2134
139. Ziolkowski PA, Henderson IR (2017) Interconnections between meiotic recombination
and sequence polymorphism in plant genomes. New Phytol 213:1022–1029
140. Li F, De Storme N, Geelen D (2017) Dynamics of male meiotic recombination frequency
during plant development using fluorescent
tagged lines in Arabidopsis thaliana. Sci Rep
7:Article number: 42535
141. Galbraith DW, Harkins KR, Knapp S (1991)
Systemic endopolyploidy in Arabidopsis thaliana. Plant Physiol 96:985–989
294
David W. Galbraith and Guiling Sun
https://doi.org/10.1186/1746-4811-4-9
123. Hirakawa T, Matsunaga S (2016) Threedimensional, live-cell imaging of chromatin
dynamics in plant nuclei using chromatin tagging
systems.
In:
Murata
M
(ed) Chromosome and genomic engineering
in plants, Methods in molecular biology, vol
1469. Humana Press, New York, pp 189–195
124. Ford K, McDonald D, Mali P (2019) Functional genomics via CRISPR-Cas. J Mol Biol
431:48–65. Special Issue: SI
125. Galbraith DW, Mauch TJ, Shields BA (1981)
Analysis of the initial stages of plant protoplast
development using 33258 Hoechst: reactivation of the cell cycle. Physiol Plant
51:380–386
126. Galbraith DW (1981) Microfluorometric
quantitation of cellulose biosynthesis by
plant protoplasts using Calcofluor White.
Physiol Plant 53:111–116
127. Galbraith DW, Shields BA (1982) The effects
of inhibitors of cell wall synthesis on tobacco
protoplast development. Physiol Plant
55:25–30
128. Galbraith DW, Lucretti S (2000) Large particle sorting. In: Radbruch A (ed) Flow cytometry and cell sorting, 2nd edn. Springer,
Berlin, pp 293–317
129. Harkins KR, Galbraith DW (1987) Factors
governing the flow cytometric analysis and
sorting of large biological particles. Cytometry 8:60–71
130. Galbraith DW (2009) Simultaneous flow
cytometric quantification of plant nuclear
DNA contents over the full range of described
angiosperm 2C values. Cytometry A
75A:692–698
131. Shapiro H (2003) Practical flow cytometry,
4th edn. John Wiley, Hoboken
132. Shendure J, Balasubramanian S, Church GM,
Gilbert W, Rogers J, Schloss JA, Waterston
RH (2017) DNA sequencing at 40: past,
present and future. Nature 550:345–353
133. Zheng GXY et al (2017) Massively parallel
digital transcriptional profiling of single cells.
Nat Commun 8:Article Number: 14049.
https://doi.org/10.1038/ncomms14049
134. Rotman N, Durbarry A, Wardle A, Yang WC,
Chaboud A, Faure JE, Berger F, Twell D
(2005) A novel class of MYB factors controls
sperm-cell formation in plants. Curr Biol
15:244–248
135. Borges F, Rui Gardner R, Lopes T, Calarco
JP, Boavida LC, Slotkin RK, Martienssen RA,
Becker JD (2012) FACS-based purification of
Arabidopsis microspores, sperm cells and vegetative nuclei. Plant Methods 8:44
136. Ingouff M, Hamamura Y, Gourgues M,
Higashiyama T, Berger F (2007) Distinct
dynamics of HISTONE3 variants between
the two fertilization products in plants. Curr
Biol 12:1032–1037
137. Misra CS, Santos MR, Rafael-Fernandes M,
Martins NP, Monteiro M, Becker JD (2019)
Transcriptomics of Arabidopsis sperm cells at
single-cell resolution. Plant Reprod 32:29–38
138. Yelina NE, Ziolkowski PA, Miller N, Zhao
XH, Kelly KA, Munoz DF, Mann DJ, Copenhaver GP, Henderson IR (2013) Highthroughput analysis of meiotic crossover frequency and interference via flow cytometry of
fluorescent pollen in Arabidopsis thaliana.
Nat Protoc 8:2119–2134
139. Ziolkowski PA, Henderson IR (2017) Interconnections between meiotic recombination
and sequence polymorphism in plant genomes. New Phytol 213:1022–1029
140. Li F, De Storme N, Geelen D (2017) Dynamics of male meiotic recombination frequency
during plant development using fluorescent
tagged lines in Arabidopsis thaliana. Sci Rep
7:Article number: 42535
141. Galbraith DW, Harkins KR, Knapp S (1991)
Systemic endopolyploidy in Arabidopsis thaliana. Plant Physiol 96:985–989
294
David W. Galbraith and Guiling Sun