Table 3.2
Type of plant oils used as a carbon source for PHA biosynthesis by R. eutropha
Type of plant oil
Plant oil
conc. (g/L)
Co-carbon
source (g/L)
Bacteria
(Class of
PHA
synthase)
Culture
time (h)
Dry cell
wt. (g/L)
PHA
content
(wt%)
Type of PHA
Corn oil (Fukui and Doi 1998)
1 0
–
R. eutropha
Wild type
(I)
72
3.6
81
P(3HB)
–
R. eutropha
PHB
-
4
recombinant
(I)
72
3.6
77
P(3HB-co-5 mol% 3HHx)
Soybean oil (Tsuge et al. 2009;
Hyakutake et al. 2011)
20
–
R. eutropha
PHB
-
4
recombinant
(IV)
72
6.0
81
P(3HB-co-0.5 mol%
3HHx)
20
–
R. eutropha
PHB
-
4
recombinant
(II)
72
1.6
57
P(3HB-co-6 mol% 3HA)
Sunflower oil (Lee et al. 2008)
5
Sodium
propionate
(5)
R. eutropha
Wild type
(I)
72
6.7
78
P(3HB-co-8 mol% 3HV)
Sodium valerate
(5)
R. eutropha
Wild type
(I)
72
5.2
80
P(3HB-co-14 mol% 3HV)
Olive oil (Lee et al. 2008)
5
Sodium
propionate
(5)
R. eutropha
Wild type
(I)
72
6.0
78
P(3HB-co-8 mol% 3HV)
(continued)
3 Development of Polyhydroxyalkanoate (PHA) and Its Copolymers as a Possible. . .
67
Type of plant oils used as a carbon source for PHA biosynthesis by R. eutropha
Type of plant oil
Plant oil
conc. (g/L)
Co-carbon
source (g/L)
Bacteria
(Class of
PHA
synthase)
Culture
time (h)
Dry cell
wt. (g/L)
PHA
content
(wt%)
Type of PHA
Corn oil (Fukui and Doi 1998)
1 0
–
R. eutropha
Wild type
(I)
72
3.6
81
P(3HB)
–
R. eutropha
PHB
-
4
recombinant
(I)
72
3.6
77
P(3HB-co-5 mol% 3HHx)
Soybean oil (Tsuge et al. 2009;
Hyakutake et al. 2011)
20
–
R. eutropha
PHB
-
4
recombinant
(IV)
72
6.0
81
P(3HB-co-0.5 mol%
3HHx)
20
–
R. eutropha
PHB
-
4
recombinant
(II)
72
1.6
57
P(3HB-co-6 mol% 3HA)
Sunflower oil (Lee et al. 2008)
5
Sodium
propionate
(5)
R. eutropha
Wild type
(I)
72
6.7
78
P(3HB-co-8 mol% 3HV)
Sodium valerate
(5)
R. eutropha
Wild type
(I)
72
5.2
80
P(3HB-co-14 mol% 3HV)
Olive oil (Lee et al. 2008)
5
Sodium
propionate
(5)
R. eutropha
Wild type
(I)
72
6.0
78
P(3HB-co-8 mol% 3HV)
(continued)
3 Development of Polyhydroxyalkanoate (PHA) and Its Copolymers as a Possible. . .
67
