analysis performed with acetaldehyde and butyraldehyde showed that the volume of
substrate binding regions was expanded. As a result, using the random-mutagenesis
method, the researchers have increased the butanol production capacity
(by increasing butanol selectivity) of the Clostridium acetobutylicum species and
managed to significantly reduce the amount of ethanol produced by the same
enzyme.
Table 9.1 Some microbial enzymes used in the production of various biofuels
Enzyme(s)
Substrate(s)
Microorganism(s) Biofuel(s)
Reference(s)
Laccase
Sugarcane
bagasse
Cyathus
stercoreus
Ethanol
Chandel et al.
(2007)
Lipase
Microalgae
Microbacterium
sp.
Biodiesel
Tripathi et al. (2014)
Cellulase
Newspaper
Cytophaga
hutchison
Bioethanol Byadgi and
Kalburgi (2016)
Cellulolytic and
xylanolytic
enzymes
Saccharum
biomasses
Sporotrichum
thermophile
BJAMDU
Bioethanol Bala and Singh
(2019)
Laccase
Saccharum
biomasses
Ganoderma
lucidum MDU-7
Bioethanol Bala and Singh
(2019)
Cellulase
Equisetum
arvense (horsetail) waste
Streptomyces
fulvissimus CKS7
Bioethanol Mihajlovski et al.
(2020)
Cellulase
Sesame seeds
residues
Bacillus cereus
Bioethanol Abada et al. (2018)
Lipase
Algae oil
Bacillus sp.
Biodiesel
Sivaramakrishnan
and Muthukumar
(2012)
Cellulase and
xylanase
Wheat straw
Trichoderma
reesei
Bioethanol Tabka et al. (2006)
Feruloyl esterase
(FAE)
Wheat straw
Aspergillus Niger Bioethanol Tabka et al. (2006)
Laccase
Wheat straw
Pycnoporus
cinnabarinus
Bioethanol Tabka et al. (2006)
α-Amylase
Corn meal
Bacillus
licheniformis
Bioethanol Mojovic et al.
(2006)
Glucoamylase
Corn meal
Aspergillus Niger Bioethanol Mojovic et al.
(2006)
Amylase
Rice water waste Bacillus
licheniformis
Bioethanol Chethana et al.
(2011)
β-Glucosidase
Bagasse and rice
straw
Pseudomonas
sp. CL3
Biobutanol Cheng et al. (2012)
Endo-glucanase
Bagasse and rice
straw
Clostridium
sp. TCW1
Biobutanol Cheng et al. (2012)
Pectinase
Microalgal
biomass
Aspergillus
aculeans
Ethanol
de Farias Silva et al.
(2018)
Laccase
Wheat straw
slurry
Pycnoporus
cinnabarinus
Bioethanol/
biogas
Moreno et al. (2013)
9 Microbial and Bioinformatics Approach in Biofuel Production
285
substrate binding regions was expanded. As a result, using the random-mutagenesis
method, the researchers have increased the butanol production capacity
(by increasing butanol selectivity) of the Clostridium acetobutylicum species and
managed to significantly reduce the amount of ethanol produced by the same
enzyme.
Table 9.1 Some microbial enzymes used in the production of various biofuels
Enzyme(s)
Substrate(s)
Microorganism(s) Biofuel(s)
Reference(s)
Laccase
Sugarcane
bagasse
Cyathus
stercoreus
Ethanol
Chandel et al.
(2007)
Lipase
Microalgae
Microbacterium
sp.
Biodiesel
Tripathi et al. (2014)
Cellulase
Newspaper
Cytophaga
hutchison
Bioethanol Byadgi and
Kalburgi (2016)
Cellulolytic and
xylanolytic
enzymes
Saccharum
biomasses
Sporotrichum
thermophile
BJAMDU
Bioethanol Bala and Singh
(2019)
Laccase
Saccharum
biomasses
Ganoderma
lucidum MDU-7
Bioethanol Bala and Singh
(2019)
Cellulase
Equisetum
arvense (horsetail) waste
Streptomyces
fulvissimus CKS7
Bioethanol Mihajlovski et al.
(2020)
Cellulase
Sesame seeds
residues
Bacillus cereus
Bioethanol Abada et al. (2018)
Lipase
Algae oil
Bacillus sp.
Biodiesel
Sivaramakrishnan
and Muthukumar
(2012)
Cellulase and
xylanase
Wheat straw
Trichoderma
reesei
Bioethanol Tabka et al. (2006)
Feruloyl esterase
(FAE)
Wheat straw
Aspergillus Niger Bioethanol Tabka et al. (2006)
Laccase
Wheat straw
Pycnoporus
cinnabarinus
Bioethanol Tabka et al. (2006)
α-Amylase
Corn meal
Bacillus
licheniformis
Bioethanol Mojovic et al.
(2006)
Glucoamylase
Corn meal
Aspergillus Niger Bioethanol Mojovic et al.
(2006)
Amylase
Rice water waste Bacillus
licheniformis
Bioethanol Chethana et al.
(2011)
β-Glucosidase
Bagasse and rice
straw
Pseudomonas
sp. CL3
Biobutanol Cheng et al. (2012)
Endo-glucanase
Bagasse and rice
straw
Clostridium
sp. TCW1
Biobutanol Cheng et al. (2012)
Pectinase
Microalgal
biomass
Aspergillus
aculeans
Ethanol
de Farias Silva et al.
(2018)
Laccase
Wheat straw
slurry
Pycnoporus
cinnabarinus
Bioethanol/
biogas
Moreno et al. (2013)
9 Microbial and Bioinformatics Approach in Biofuel Production
285