Chapter 6
Engineering of Zymomonas mobilis
for Enhanced Biofuel Production
Muneeba Khalid, Nasheen Rubab, Wajiha Afzal, Muhammad Irfan,
Misbah Ghazanfar, Hafiz Abdullah Shakir, Muhammad Khan, Shaukat Ali,
and Marcelo Franco
Abstract Zymomonas mobilis strains are examined as the model organism in the
industries because of having many potential advantages. Different research showed
that different strains of Z. mobilis produced high amount of ethanol and the sugar
because it can easily utilize xylose and arabinose in addition to glucose. We can
improve the strains of Z. mobilis such as by adaptive laboratory evolution (ALE) by
many methods. It is a very important method for the improvement of different
attributes of common industrial strains. These strains have used as advanced
model organism for genetic method and inverse metabolic engineering. The ED
pathway of Z. mobilis gives an alternative way for the production of bio refineries
and valuable byproducts like sorbitol, succinic acid, levan, and isobutanol. The
metabolic engineering using Z. mobilis gives advanced biofuel production. The
techniques adopted for strain improvement and future guidelines for this were
discussed.
Keywords Engineering · Zymomonas mobilis · Biofuels · Fermentation
M. Khalid · N. Rubab · W. Afzal · M. Irfan (*) · M. Ghazanfar
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
e-mail: irfan.ashraf@uos.edu.pk
H. A. Shakir · M. Khan
Department of Zoology, University of the Punjab, Lahore, Pakistan
S. Ali
Department of Zoology, Government College University, Lahore, Pakistan
M. Franco
Department of Exact Sciences and Technology, State University of Santa Cruz (UESC), Ilhéus,
Brazil
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
M. Srivastava et al. (eds.), Bioenergy Research: Basic and Advanced Concepts, Clean
Energy Production Technologies, https://doi.org/10.1007/978-981-33-4611-6_6
155
Engineering of Zymomonas mobilis
for Enhanced Biofuel Production
Muneeba Khalid, Nasheen Rubab, Wajiha Afzal, Muhammad Irfan,
Misbah Ghazanfar, Hafiz Abdullah Shakir, Muhammad Khan, Shaukat Ali,
and Marcelo Franco
Abstract Zymomonas mobilis strains are examined as the model organism in the
industries because of having many potential advantages. Different research showed
that different strains of Z. mobilis produced high amount of ethanol and the sugar
because it can easily utilize xylose and arabinose in addition to glucose. We can
improve the strains of Z. mobilis such as by adaptive laboratory evolution (ALE) by
many methods. It is a very important method for the improvement of different
attributes of common industrial strains. These strains have used as advanced
model organism for genetic method and inverse metabolic engineering. The ED
pathway of Z. mobilis gives an alternative way for the production of bio refineries
and valuable byproducts like sorbitol, succinic acid, levan, and isobutanol. The
metabolic engineering using Z. mobilis gives advanced biofuel production. The
techniques adopted for strain improvement and future guidelines for this were
discussed.
Keywords Engineering · Zymomonas mobilis · Biofuels · Fermentation
M. Khalid · N. Rubab · W. Afzal · M. Irfan (*) · M. Ghazanfar
Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
e-mail: irfan.ashraf@uos.edu.pk
H. A. Shakir · M. Khan
Department of Zoology, University of the Punjab, Lahore, Pakistan
S. Ali
Department of Zoology, Government College University, Lahore, Pakistan
M. Franco
Department of Exact Sciences and Technology, State University of Santa Cruz (UESC), Ilhéus,
Brazil
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
M. Srivastava et al. (eds.), Bioenergy Research: Basic and Advanced Concepts, Clean
Energy Production Technologies, https://doi.org/10.1007/978-981-33-4611-6_6
155