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56. Hamada S, Ishiyama K, Choi S-B et al (2003)
The transport of prolamine RNAs to prolamine
protein bodies in living rice endosperm cells.
Plant Cell 15:2253–2264
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targeting of mobile mRNAs to plasmodesmata
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Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.
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RNA 23:134–141
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62. Vera M, Tutucci E, Singer RH (2019) Imaging
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using an optimized MS2-MCP system. Methods Mol Biol 2038:3–20
63. Tutucci E, Vera M, Singer RH (2018) SinglemRNA detection in living S. cerevisiae using a
re-engineered MS2 system. Nat Protoc
13:2268–2296
64. Curtis MD, Grossniklaus U (2003) A gateway
cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol
133:462–469
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66. Kapila J, De Rycke R, Van Montagu M, Angenon G (1997) An Agrobacterium-mediated
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67. Voinnet O, Rivas S, Mestre P, Baulcombe D
(2003) An enhanced transient expression system in plants based on suppression of gene
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68. Pen ˜a EJ, Heinlein M (2016) In vivo RNA visualization in plants using MS2 tagging. Methods
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120
Eduardo Jose ´ Pen ˜ a and Manfred Heinlein
8:1161
54. Tilsner J (2015) Techniques for RNA in vivo
imaging in plants. J Microsc 258:1–5
55. Tutucci E, Livingston NM, Singer RH, Wu B
(2018) Imaging mRNA in vivo, from birth to
death. Annu Rev Biophys 47:85–106
56. Hamada S, Ishiyama K, Choi S-B et al (2003)
The transport of prolamine RNAs to prolamine
protein bodies in living rice endosperm cells.
Plant Cell 15:2253–2264
57. Luo K-R, Huang N-C, Yu T-S (2018) Selective
targeting of mobile mRNAs to plasmodesmata
for cell-to-cell movement. Plant Physiol
177:604–614
58. Fusco D, Accornero N, Lavoie B et al (2003)
Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.
Curr Biol 13:161–167
59. Heinrich S, Sidler CL, Azzalin CM, Weis K
(2017) Stem-loop RNA labeling can affect
nuclear and cytoplasmic mRNA processing.
RNA 23:134–141
60. Garcia JF, Parker R (2015) MS2 coat proteins
bound to yeast mRNAs block 5
0 to 3
0 degradation and trap mRNA decay products: implications for the localization of mRNAs by
MS2-MCP system. RNA 21:1393–1395
61. Tutucci E, Vera M, Biswas J et al (2018) An
improved MS2 system for accurate reporting of
the mRNA life cycle. Nat Methods 15:81–89
62. Vera M, Tutucci E, Singer RH (2019) Imaging
single mRNA molecules in mammalian cells
using an optimized MS2-MCP system. Methods Mol Biol 2038:3–20
63. Tutucci E, Vera M, Singer RH (2018) SinglemRNA detection in living S. cerevisiae using a
re-engineered MS2 system. Nat Protoc
13:2268–2296
64. Curtis MD, Grossniklaus U (2003) A gateway
cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol
133:462–469
65. Hellens R, Mullineaux P, Klee H (2000) Technical focus: a guide to Agrobacterium binary Ti
vectors. Trends Plant Sci 5:446–451
66. Kapila J, De Rycke R, Van Montagu M, Angenon G (1997) An Agrobacterium-mediated
transient gene expression system for intact
leaves. Plant Sci 122:101–108
67. Voinnet O, Rivas S, Mestre P, Baulcombe D
(2003) An enhanced transient expression system in plants based on suppression of gene
silencing by the p19 protein of tomato bushy
stunt virus. Plant J 33:949–956
68. Pen ˜a EJ, Heinlein M (2016) In vivo RNA visualization in plants using MS2 tagging. Methods
Enzymol 572:105–122
120
Eduardo Jose ´ Pen ˜ a and Manfred Heinlein