An example of iSERS imaging is shown in Fig. 6.10b. Here, gold nanoparticles
covered with a Raman reporter molecule and ethylene glycol spacer (mixture of
4-NTB-MEG-OH and 4-NTB-TEG-COOH according to protocol in [195]) were
conjugated with an anti-actin smooth muscle cells (SMCs) antibody labelled by a
cy3 fluorophore. This iSERS probes allowed detecting one of the most important
factors determining the formation of the atherosclerotic plaque in the murine brachiocephalic artery.
References
1. Atkins PW, Barron LD (1969) Rayleigh scattering of polarized photons by molecules. Mol
Phys 16:453–466
2. Barron LD, Buckingham AD (1971) Rayleigh and Raman scattering from optically active
molecules. Mol Phys 20:1111–1119
3. Barron LD, Bogaard MP, Buckingham AD (1973) Raman scattering of circularly polarized
light by optically active molecules. J Am Chem Soc 95:603–605
4. Hecht L, Barron LD, Blanch EW, Bell AF, Day LA, Ziegler LD (1999) Raman optical
activity instrument for studies of biopolymer structure and dynamics. J Raman Spectrosc
30:815–825
5. Hug W (2003) Virtual enantiomers as the solution of optical activity’s deterministic offset
problem. Appl Spectrosc 57:1–13
6. Hug W (2010) Raman optical activity, spectrometers A2. In: Lindon JC (ed) BT—
encyclopedia of spectroscopy and spectrometry, 2nd edn. Academic Press, Oxford,
pp 2387–2396
7. Kapitán J, Barron LD, Hecht L (2015) A novel Raman optical activity instrument operating
in the deep ultraviolet spectral region. J Raman Spectrosc 46:392–399
8. Kubota K, Shingae T, Foster ND, Kumauchi M, Hoff WD, Unno M (2013) Active site
structure of photoactive yellow protein with a locked chromophore analogue revealed by
near-infrared Raman optical activity. J Phys Chem Lett 4:3031–3038
9. Profant V, Pazderková M, Pazderka T, Maloň P, Baumruk V (2014) Relative intensity
correction of Raman optical activity spectra facilitates extending the spectral region.
J Raman Spectrosc 45:603–609
10. Shingae T, Kubota K, Kumauchi M, Tokunaga F, Unno M (2013) Raman optical activity
probing structural deformations of the 4-hydroxycinnamyl chromophore in photoactive
yellow protein. J Phys Chem Lett 4:1322–1327
11. Unno M, Kikukawa T, Kumauchi M, Kamo N (2013) Exploring the active site structure of a
photoreceptor protein by Raman optical activity. J Phys Chem B 117:1321–1325
12. Yamamoto S, Watarai H (2010) Incident circularly polarized Raman optical activity
spectrometer based on circularity conversion method. J Raman Spectrosc 41:1664–1669
13. Zhang Y, Wang P, Jia G, Cheng F, Li C (2017) A short-wavelength Raman optical activity
spectrometer with laser source at 457 nm for the characterization of chiral molecules. Appl
Spectrosc 71:2211–2217
14. Barron LD, Hecht L, Blanch EW, Bell AF (2000) Solution structure and dynamics of
biomolecules from Raman optical activity. Prog Biophys Mol Biol 73:1–49
15. Blanch E (2003) Vibrational Raman optical activity of proteins, nucleic acids, and viruses.
Methods 29:196–209
16. Barron LD, Hecht L, McColl IH, Blanch EW (2004) Raman optical activity comes of age.
Mol Phys 102:731–744
188
K. Czamara et al.
covered with a Raman reporter molecule and ethylene glycol spacer (mixture of
4-NTB-MEG-OH and 4-NTB-TEG-COOH according to protocol in [195]) were
conjugated with an anti-actin smooth muscle cells (SMCs) antibody labelled by a
cy3 fluorophore. This iSERS probes allowed detecting one of the most important
factors determining the formation of the atherosclerotic plaque in the murine brachiocephalic artery.
References
1. Atkins PW, Barron LD (1969) Rayleigh scattering of polarized photons by molecules. Mol
Phys 16:453–466
2. Barron LD, Buckingham AD (1971) Rayleigh and Raman scattering from optically active
molecules. Mol Phys 20:1111–1119
3. Barron LD, Bogaard MP, Buckingham AD (1973) Raman scattering of circularly polarized
light by optically active molecules. J Am Chem Soc 95:603–605
4. Hecht L, Barron LD, Blanch EW, Bell AF, Day LA, Ziegler LD (1999) Raman optical
activity instrument for studies of biopolymer structure and dynamics. J Raman Spectrosc
30:815–825
5. Hug W (2003) Virtual enantiomers as the solution of optical activity’s deterministic offset
problem. Appl Spectrosc 57:1–13
6. Hug W (2010) Raman optical activity, spectrometers A2. In: Lindon JC (ed) BT—
encyclopedia of spectroscopy and spectrometry, 2nd edn. Academic Press, Oxford,
pp 2387–2396
7. Kapitán J, Barron LD, Hecht L (2015) A novel Raman optical activity instrument operating
in the deep ultraviolet spectral region. J Raman Spectrosc 46:392–399
8. Kubota K, Shingae T, Foster ND, Kumauchi M, Hoff WD, Unno M (2013) Active site
structure of photoactive yellow protein with a locked chromophore analogue revealed by
near-infrared Raman optical activity. J Phys Chem Lett 4:3031–3038
9. Profant V, Pazderková M, Pazderka T, Maloň P, Baumruk V (2014) Relative intensity
correction of Raman optical activity spectra facilitates extending the spectral region.
J Raman Spectrosc 45:603–609
10. Shingae T, Kubota K, Kumauchi M, Tokunaga F, Unno M (2013) Raman optical activity
probing structural deformations of the 4-hydroxycinnamyl chromophore in photoactive
yellow protein. J Phys Chem Lett 4:1322–1327
11. Unno M, Kikukawa T, Kumauchi M, Kamo N (2013) Exploring the active site structure of a
photoreceptor protein by Raman optical activity. J Phys Chem B 117:1321–1325
12. Yamamoto S, Watarai H (2010) Incident circularly polarized Raman optical activity
spectrometer based on circularity conversion method. J Raman Spectrosc 41:1664–1669
13. Zhang Y, Wang P, Jia G, Cheng F, Li C (2017) A short-wavelength Raman optical activity
spectrometer with laser source at 457 nm for the characterization of chiral molecules. Appl
Spectrosc 71:2211–2217
14. Barron LD, Hecht L, Blanch EW, Bell AF (2000) Solution structure and dynamics of
biomolecules from Raman optical activity. Prog Biophys Mol Biol 73:1–49
15. Blanch E (2003) Vibrational Raman optical activity of proteins, nucleic acids, and viruses.
Methods 29:196–209
16. Barron LD, Hecht L, McColl IH, Blanch EW (2004) Raman optical activity comes of age.
Mol Phys 102:731–744
188
K. Czamara et al.