Chapter 6
Small and Large Molecules
Investigated by Raman Spectroscopy
From Conformational Study
to Biomedical Applications
Krzysztof Czamara, Ewelina Szafraniec, Ewelina Wiercigroch,
Szymon Tott, Grzegorz Zając, Ewa Machalska, Monika Dudek,
Dominika Augustynska, Kamilla Malek, Agnieszka Kaczor
and Malgorzata Baranska
Abstract This chapter presents selected techniques of Raman spectroscopy, i.e.
Raman imaging, Raman optical activity (ROA), and surface-enhanced Raman
spectroscopy (SERS), and gives an overview on their biomedical applications. The
current state of the art in the research on chiroptical compounds of biomedical
importance, as well as the study on early apoptosis and inflammation processes
occuring in the endothelium, is presented. The pathophysiology of the endothelium
is discussed based on the example of Raman imaging results for primary cells and
cell cultures. Moreover, the comparison of classical Raman imaging, application of
optical fiber probes, and immuno-SERS nanosensors in detection of marker proteins
in ex vivo studies is discussed.
6.1 Molecules of Biomedical Importance—Structure
and Interactions
In this section, we focus on principles of two Raman techniques which rely on
registration of light scattering generated due to interactions with optically active
chemical individuals (ROA, Raman optical activity) and with roughened metal
surfaces (SERS, surface-enhanced Raman spectroscopy). Active modes observed in
K. Czamara Á E. Szafraniec Á E. Wiercigroch Á S. Tott Á G. Zając
E. Machalska Á M. Dudek Á K. Malek Á A. Kaczor Á M. Baranska (&)
Faculty of Chemistry, Jagiellonian University, Krakow, Poland
e-mail: baranska@chemia.uj.edu.pl
K. Czamara Á D. Augustynska Á K. Malek Á A. Kaczor Á M. Baranska
Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University,
Krakow, Poland
© Springer Nature Switzerland AG 2019
A. Koleżyński and M. Król (eds.), Molecular Spectroscopy—Experiment
and Theory, Challenges and Advances in Computational Chemistry
and Physics 26, https://doi.org/10.1007/978-3-030-01355-4_6
161
Small and Large Molecules
Investigated by Raman Spectroscopy
From Conformational Study
to Biomedical Applications
Krzysztof Czamara, Ewelina Szafraniec, Ewelina Wiercigroch,
Szymon Tott, Grzegorz Zając, Ewa Machalska, Monika Dudek,
Dominika Augustynska, Kamilla Malek, Agnieszka Kaczor
and Malgorzata Baranska
Abstract This chapter presents selected techniques of Raman spectroscopy, i.e.
Raman imaging, Raman optical activity (ROA), and surface-enhanced Raman
spectroscopy (SERS), and gives an overview on their biomedical applications. The
current state of the art in the research on chiroptical compounds of biomedical
importance, as well as the study on early apoptosis and inflammation processes
occuring in the endothelium, is presented. The pathophysiology of the endothelium
is discussed based on the example of Raman imaging results for primary cells and
cell cultures. Moreover, the comparison of classical Raman imaging, application of
optical fiber probes, and immuno-SERS nanosensors in detection of marker proteins
in ex vivo studies is discussed.
6.1 Molecules of Biomedical Importance—Structure
and Interactions
In this section, we focus on principles of two Raman techniques which rely on
registration of light scattering generated due to interactions with optically active
chemical individuals (ROA, Raman optical activity) and with roughened metal
surfaces (SERS, surface-enhanced Raman spectroscopy). Active modes observed in
K. Czamara Á E. Szafraniec Á E. Wiercigroch Á S. Tott Á G. Zając
E. Machalska Á M. Dudek Á K. Malek Á A. Kaczor Á M. Baranska (&)
Faculty of Chemistry, Jagiellonian University, Krakow, Poland
e-mail: baranska@chemia.uj.edu.pl
K. Czamara Á D. Augustynska Á K. Malek Á A. Kaczor Á M. Baranska
Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University,
Krakow, Poland
© Springer Nature Switzerland AG 2019
A. Koleżyński and M. Król (eds.), Molecular Spectroscopy—Experiment
and Theory, Challenges and Advances in Computational Chemistry
and Physics 26, https://doi.org/10.1007/978-3-030-01355-4_6
161