Fig. 11
Schematic representation of detection of biomolecules on bio-chemically treated silicon substrates using APTES and glutaraldehyde linker. The method
involves the following: (a) silicon substrates cleaning (piranha cleaning, oxygen plasma treatment, or UV/ozone treatment); (b) generation of silane layer using
optimized APTES (2%, v/v) solution; (c) treatment with glutaraldehyde solution; (d) immobilization of capture antibodies; (e) addition of antigen biomolecules;
and (f) detection of antigens using FITC-tagged detection antibodies. Reprinted with permission from [41]
334
B. Della Ventura et al.
Schematic representation of detection of biomolecules on bio-chemically treated silicon substrates using APTES and glutaraldehyde linker. The method
involves the following: (a) silicon substrates cleaning (piranha cleaning, oxygen plasma treatment, or UV/ozone treatment); (b) generation of silane layer using
optimized APTES (2%, v/v) solution; (c) treatment with glutaraldehyde solution; (d) immobilization of capture antibodies; (e) addition of antigen biomolecules;
and (f) detection of antigens using FITC-tagged detection antibodies. Reprinted with permission from [41]
334
B. Della Ventura et al.