Vol.:(0123456789)
Topics in Current Chemistry (2020) 378:13
https://doi.org/10.1007/s41061-019-0277-9
1 3
REVIEW
DNA–Iron Oxide Nanoparticles Conjugates: Functional
Magnetic Nanoplatforms in Biomedical Applications
José Raúl Sosa‑Acosta
1,2
· Claudia Iriarte‑Mesa
1
· Greter A. Ortega
1,3
·
Alicia M. Díaz‑García
1
Received: 24 July 2019 / Accepted: 20 December 2019 / Published online: 10 January 2020
© Springer Nature Switzerland AG 2020
Abstract
The use of magnetic nanoparticles (MNPs), such as iron oxide nanoparticles
(IONPs), in biomedicine is considered to be a valuable alternative to the more traditional materials due to their chemical stability, cost-effectiveness, surface functionalization, and the possibility to selectively attach and transport targeted species
to the desired location under a magnetic field. One of the many main applications
of MNPs is DNA separation, which enables genetic material manipulation; consequently, MNPs are used in numerous biotechnological methods, such as gene transfection and molecular recognition systems. In addition, the interaction between
the surfaces of MNPs and DNA molecules and the magnetic nature of the resulting composite have facilitated the development of safe and effective gene delivery
vectors to treat significant diseases, such as cancer and neurological disorders. Furthermore, the special recognition properties of nucleic acids based on the binding
capacity of DNA and the magnetic behavior of the nanoparticles allowing magnetic
separation and concentration of analytes have led to the development of biosensors
and diagnostic assays; however, both of these applications face important challenges
in terms of the improvement of selective nanocarriers and biosensing capacity. In
this review, we discuss some aspects of the properties and surface functionalization
of MNPs, the interactions between DNA and IONPs, the preparation of DNA nanoplatforms and their biotechnological applications, such as the magnetic separation
of DNA, magnetofection, preparation of DNA vaccines, and molecular recognition
tools.
Keywords Magnetic nanoparticles · DNA conjugation · Nucleic acid separation ·
DNA-based therapeutics
* Alicia M. Díaz-García
adg1959@gmail.com
Extended author information available on the last page of the article
19
Reprinted from the journal
Chapter 2 was originally published as Sosa‑Acosta, J. R., Iriarte‑Mesa, C., Ortega, G. A. & Díaz‑García,
A. M. Topics in Current Chemistry (2020) 378: 13. https://doi.org/10.1007/s41061-019-0277-9.
Topics in Current Chemistry (2020) 378:13
https://doi.org/10.1007/s41061-019-0277-9
1 3
REVIEW
DNA–Iron Oxide Nanoparticles Conjugates: Functional
Magnetic Nanoplatforms in Biomedical Applications
José Raúl Sosa‑Acosta
1,2
· Claudia Iriarte‑Mesa
1
· Greter A. Ortega
1,3
·
Alicia M. Díaz‑García
1
Received: 24 July 2019 / Accepted: 20 December 2019 / Published online: 10 January 2020
© Springer Nature Switzerland AG 2020
Abstract
The use of magnetic nanoparticles (MNPs), such as iron oxide nanoparticles
(IONPs), in biomedicine is considered to be a valuable alternative to the more traditional materials due to their chemical stability, cost-effectiveness, surface functionalization, and the possibility to selectively attach and transport targeted species
to the desired location under a magnetic field. One of the many main applications
of MNPs is DNA separation, which enables genetic material manipulation; consequently, MNPs are used in numerous biotechnological methods, such as gene transfection and molecular recognition systems. In addition, the interaction between
the surfaces of MNPs and DNA molecules and the magnetic nature of the resulting composite have facilitated the development of safe and effective gene delivery
vectors to treat significant diseases, such as cancer and neurological disorders. Furthermore, the special recognition properties of nucleic acids based on the binding
capacity of DNA and the magnetic behavior of the nanoparticles allowing magnetic
separation and concentration of analytes have led to the development of biosensors
and diagnostic assays; however, both of these applications face important challenges
in terms of the improvement of selective nanocarriers and biosensing capacity. In
this review, we discuss some aspects of the properties and surface functionalization
of MNPs, the interactions between DNA and IONPs, the preparation of DNA nanoplatforms and their biotechnological applications, such as the magnetic separation
of DNA, magnetofection, preparation of DNA vaccines, and molecular recognition
tools.
Keywords Magnetic nanoparticles · DNA conjugation · Nucleic acid separation ·
DNA-based therapeutics
* Alicia M. Díaz-García
adg1959@gmail.com
Extended author information available on the last page of the article
19
Reprinted from the journal
Chapter 2 was originally published as Sosa‑Acosta, J. R., Iriarte‑Mesa, C., Ortega, G. A. & Díaz‑García,
A. M. Topics in Current Chemistry (2020) 378: 13. https://doi.org/10.1007/s41061-019-0277-9.