76. Horikawa K et al (2010) Spontaneous network activity visualized by ultrasensitive Ca(2+)
indicators, yellow Cameleon-Nano. Nat Methods 7(9):729–732
77. Nagai T et al (2004) Expanded dynamic range of fluorescent indicators for Ca(2+) by
circularly permuted yellow fluorescent proteins. Proc Natl Acad Sci U S A 101
(29):10554–10559
78. Palmer AE et al (2004) Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed
with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A 101
(50):17404–17409
79. Palmer AE et al (2006) Ca2+ indicators based on computationally redesigned calmodulinpeptide pairs. Chem Biol 13(5):521–530
80. Mank M et al (2008) A genetically encoded calcium indicator for chronic in vivo two-photon
imaging. Nat Methods 5(9):805–811
81. Vinkenborg JL et al (2009) Genetically encoded FRET sensors to monitor intracellular Zn2+
homeostasis. Nat Methods 6(10):737–740
82. Evers TH et al (2007) Ratiometric detection of Zn(II) using chelating fluorescent protein
chimeras. J Mol Biol 374(2):411–425
83. Qin Y et al (2011) Measuring steady-state and dynamic endoplasmic reticulum and Golgi
Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A 108(18):7351–7356
84. Emmanouilidou E et al (1999) Imaging Ca2+ concentration changes at the secretory vesicle
surface with a recombinant targeted cameleon. Curr Biol 9(16):915–918
85. Palmer AE, Tsien RY (2006) Measuring calcium signaling using genetically targetable
fluorescent indicators. Nat Protoc 1(3):1057–1065
86. Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of
protein interactions in living cells. Annu Rev Biophys 37:465–487
87. Kerppola TK (2008) Bimolecular fluorescence complementation: visualization of molecular
interactions in living cells. Methods Cell Biol 85:431–470
88. Kodama Y, Hu CD (2012) Bimolecular fluorescence complementation (BiFC): a 5-year
update and future perspectives. Biotechniques 53(5):285–298
89. Kerppola TK (2006) Visualization of molecular interactions by fluorescence complementation. Nat Rev Mol Cell Biol 7(6):449–456
90. Magliery TJ et al (2005) Detecting protein-protein interactions with a green fluorescent protein
fragment reassembly trap: scope and mechanism. J Am Chem Soc 127(1):146–157
91. Hu CD, Chinenov Y, Kerppola TK (2002) Visualization of interactions among bZIP and Rel
family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9
(4):789–798
92. Hu CD, Kerppola TK (2003) Simultaneous visualization of multiple protein interactions in
living cells using multicolor fluorescence complementation analysis. Nat Biotechnol 21
(5):539–545
93. Shyu YJ et al (2006) Identification of new fluorescent protein fragments for bimolecular
fluorescence complementation analysis under physiological conditions. Biotechniques 40
(1):61–66
94. Waadt R et al (2008) Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J 56(3):505–516
95. Fan JY et al (2008) Split mCherry as a new red bimolecular fluorescence complementation
system for visualizing protein-protein interactions in living cells. Biochem Biophys Res
Commun 367(1):47–53
96. Kodama Y, Wada M (2009) Simultaneous visualization of two protein complexes in a single
plant cell using multicolor fluorescence complementation analysis. Plant Mol Biol 70
(1–2):211–217
97. Chu J et al (2009) A novel far-red bimolecular fluorescence complementation system that
allows for efficient visualization of protein interactions under physiological conditions.
Biosens Bioelectron 25(1):234–239
Applications of Fluorescent Protein-Based Sensors in Bioimaging
179
indicators, yellow Cameleon-Nano. Nat Methods 7(9):729–732
77. Nagai T et al (2004) Expanded dynamic range of fluorescent indicators for Ca(2+) by
circularly permuted yellow fluorescent proteins. Proc Natl Acad Sci U S A 101
(29):10554–10559
78. Palmer AE et al (2004) Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed
with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A 101
(50):17404–17409
79. Palmer AE et al (2006) Ca2+ indicators based on computationally redesigned calmodulinpeptide pairs. Chem Biol 13(5):521–530
80. Mank M et al (2008) A genetically encoded calcium indicator for chronic in vivo two-photon
imaging. Nat Methods 5(9):805–811
81. Vinkenborg JL et al (2009) Genetically encoded FRET sensors to monitor intracellular Zn2+
homeostasis. Nat Methods 6(10):737–740
82. Evers TH et al (2007) Ratiometric detection of Zn(II) using chelating fluorescent protein
chimeras. J Mol Biol 374(2):411–425
83. Qin Y et al (2011) Measuring steady-state and dynamic endoplasmic reticulum and Golgi
Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A 108(18):7351–7356
84. Emmanouilidou E et al (1999) Imaging Ca2+ concentration changes at the secretory vesicle
surface with a recombinant targeted cameleon. Curr Biol 9(16):915–918
85. Palmer AE, Tsien RY (2006) Measuring calcium signaling using genetically targetable
fluorescent indicators. Nat Protoc 1(3):1057–1065
86. Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of
protein interactions in living cells. Annu Rev Biophys 37:465–487
87. Kerppola TK (2008) Bimolecular fluorescence complementation: visualization of molecular
interactions in living cells. Methods Cell Biol 85:431–470
88. Kodama Y, Hu CD (2012) Bimolecular fluorescence complementation (BiFC): a 5-year
update and future perspectives. Biotechniques 53(5):285–298
89. Kerppola TK (2006) Visualization of molecular interactions by fluorescence complementation. Nat Rev Mol Cell Biol 7(6):449–456
90. Magliery TJ et al (2005) Detecting protein-protein interactions with a green fluorescent protein
fragment reassembly trap: scope and mechanism. J Am Chem Soc 127(1):146–157
91. Hu CD, Chinenov Y, Kerppola TK (2002) Visualization of interactions among bZIP and Rel
family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9
(4):789–798
92. Hu CD, Kerppola TK (2003) Simultaneous visualization of multiple protein interactions in
living cells using multicolor fluorescence complementation analysis. Nat Biotechnol 21
(5):539–545
93. Shyu YJ et al (2006) Identification of new fluorescent protein fragments for bimolecular
fluorescence complementation analysis under physiological conditions. Biotechniques 40
(1):61–66
94. Waadt R et al (2008) Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J 56(3):505–516
95. Fan JY et al (2008) Split mCherry as a new red bimolecular fluorescence complementation
system for visualizing protein-protein interactions in living cells. Biochem Biophys Res
Commun 367(1):47–53
96. Kodama Y, Wada M (2009) Simultaneous visualization of two protein complexes in a single
plant cell using multicolor fluorescence complementation analysis. Plant Mol Biol 70
(1–2):211–217
97. Chu J et al (2009) A novel far-red bimolecular fluorescence complementation system that
allows for efficient visualization of protein interactions under physiological conditions.
Biosens Bioelectron 25(1):234–239
Applications of Fluorescent Protein-Based Sensors in Bioimaging
179