Fig. 3
Different types of FP-based
fluorescent sensors: (a) circularly permuted FP fused to specific sensitive domain(s). (b) Two FPs of different colors are
fused to sensitive domain(s); those conformational changes influence the efficiency of FRET between these FPs. (c) Intrinsically sensitive FP undergoes
ion-dependent changes in spectral properties (brightness or excitation-emission wavelengths), e.g., pH. (d) Translocation sensors: FP fused to specific protein
domains demonstrates environmentally dependent changes in intracellular localization
Applications of Fluorescent Protein-Based Sensors in Bioimaging
157
Different types of FP-based
fluorescent sensors: (a) circularly permuted FP fused to specific sensitive domain(s). (b) Two FPs of different colors are
fused to sensitive domain(s); those conformational changes influence the efficiency of FRET between these FPs. (c) Intrinsically sensitive FP undergoes
ion-dependent changes in spectral properties (brightness or excitation-emission wavelengths), e.g., pH. (d) Translocation sensors: FP fused to specific protein
domains demonstrates environmentally dependent changes in intracellular localization
Applications of Fluorescent Protein-Based Sensors in Bioimaging
157