41
microsphere-based fiber-optic biosensors, and surface plasmon resonance. It should
be noted that although fluorescence detection is the most popular, surface plasmon
resonance (SPR)-based immunoassay formats with surface imaging capabilities are
growing in popularity [80–86]. Multiplexing, or simultaneous detection of multiple
analytes, is one of the most important prerequisites for biothreat agent detection. As
mentioned in our introduction, the presented work is more a “Comptes Rendus” and
an introduction to this series of sensing measuring devices used for the detection of
biological warfare agents that were presented to the participants of this NATO-ASI
meeting.
Fig. 2.22 Sensorgram showing different steps [(1) Baseline (2) EDCNHS activation (3) Washing
(4) Antibody coupling (5) Washing (6) Deactivation (7) Washing (8) Regeneration and (9) Back to
baseline] involved in the (a) immobilization of BoNT/A antibody (1: 500 dilution); (b) immobilization of SV on CM5 chip [84]
2 Detection of Biological Warfare Agents Using Biosensors
microsphere-based fiber-optic biosensors, and surface plasmon resonance. It should
be noted that although fluorescence detection is the most popular, surface plasmon
resonance (SPR)-based immunoassay formats with surface imaging capabilities are
growing in popularity [80–86]. Multiplexing, or simultaneous detection of multiple
analytes, is one of the most important prerequisites for biothreat agent detection. As
mentioned in our introduction, the presented work is more a “Comptes Rendus” and
an introduction to this series of sensing measuring devices used for the detection of
biological warfare agents that were presented to the participants of this NATO-ASI
meeting.
Fig. 2.22 Sensorgram showing different steps [(1) Baseline (2) EDCNHS activation (3) Washing
(4) Antibody coupling (5) Washing (6) Deactivation (7) Washing (8) Regeneration and (9) Back to
baseline] involved in the (a) immobilization of BoNT/A antibody (1: 500 dilution); (b) immobilization of SV on CM5 chip [84]
2 Detection of Biological Warfare Agents Using Biosensors