243
© Springer Nature B.V. 2020
G. Sindona et al. (eds.), Toxic Chemical and Biological Agents, NATO Science
for Peace and Security Series A: Chemistry and Biology,
https://doi.org/10.1007/978-94-024-2041-8_22
Chapter 22
A Novel Origami Paper-Based
Chemiluminescent Biosensor Integrated
with a Smartphone Device
for Organophosphorus Compounds
Detection
Laura Montali, Maria Maddalena Calabretta, Antonia Lopreside,
M. Guardigli, Elisa Michelini, and Aldo Roda
Abstract The study presents the development and optimization of a novel origami
paper-based chemiluminescent (CL) biosensor for measuring the acetylcholinesterase activity detection and its implementation into portable analytical devices as
proof-of-principle of low-cost point-of-care applications.
Global security threats have become a major worldwide concern, and their early and
sensitive detection represents a major challenge to current detection technologies.
The routine monitoring of water, food, and the environment for chemical and biological threat agents is often hampered by the fact that available techniques usually
require clean samples and sophisticated equipment based on high-performance liquid chromatography-tandem mass spectrometry and are thus unsuitable for realtime, cost-effective, and on-field routine monitoring.
The possibility of implementing enzymatic assays with bio-chemiluminescence
detection in smartphones has been very successful over the years, as well as the possibility of creating ad hoc analytical devices manufactured with an easy and economical 3D printing technology. Here, we report the development and optimization
L. Montali (*) · M. M. Calabretta · A. Lopreside · M. Guardigli
Department of Chemistry “G. Ciamician”, University of Bologna, Bologna, Italy
e-mail: laura.montali2@unibo.it; antonia.lopreside2@unibo.it
E. Michelini · A. Roda
Department of Chemistry “G. Ciamician”, University of Bologna, Bologna, Italy
INBB, Istituto Nazionale di Biostrutture e Biosistemi, Rome, Italy
© Springer Nature B.V. 2020
G. Sindona et al. (eds.), Toxic Chemical and Biological Agents, NATO Science
for Peace and Security Series A: Chemistry and Biology,
https://doi.org/10.1007/978-94-024-2041-8_22
Chapter 22
A Novel Origami Paper-Based
Chemiluminescent Biosensor Integrated
with a Smartphone Device
for Organophosphorus Compounds
Detection
Laura Montali, Maria Maddalena Calabretta, Antonia Lopreside,
M. Guardigli, Elisa Michelini, and Aldo Roda
Abstract The study presents the development and optimization of a novel origami
paper-based chemiluminescent (CL) biosensor for measuring the acetylcholinesterase activity detection and its implementation into portable analytical devices as
proof-of-principle of low-cost point-of-care applications.
Global security threats have become a major worldwide concern, and their early and
sensitive detection represents a major challenge to current detection technologies.
The routine monitoring of water, food, and the environment for chemical and biological threat agents is often hampered by the fact that available techniques usually
require clean samples and sophisticated equipment based on high-performance liquid chromatography-tandem mass spectrometry and are thus unsuitable for realtime, cost-effective, and on-field routine monitoring.
The possibility of implementing enzymatic assays with bio-chemiluminescence
detection in smartphones has been very successful over the years, as well as the possibility of creating ad hoc analytical devices manufactured with an easy and economical 3D printing technology. Here, we report the development and optimization
L. Montali (*) · M. M. Calabretta · A. Lopreside · M. Guardigli
Department of Chemistry “G. Ciamician”, University of Bologna, Bologna, Italy
e-mail: laura.montali2@unibo.it; antonia.lopreside2@unibo.it
E. Michelini · A. Roda
Department of Chemistry “G. Ciamician”, University of Bologna, Bologna, Italy
INBB, Istituto Nazionale di Biostrutture e Biosistemi, Rome, Italy