195
carbohydrate moiety was assigned using the nomenclature introduced by Domon
and Costello, as A, B, C, X, Y, and Z [75]. An example of the mass spectrometry
characterization of neoglycoconjugate vaccines is discussed in the next section.
Fig. 11.5 The progress of conjugation of the hexasaccharide of Vibrio cholerae serotype Ogawa
(exact mass, 1780.79 Da) and BSA (molecular mass 66,430 Da) as revealed by monitoring the
reaction by SELDI-TOF MS. Spectrum A was taken at the onset of the reaction (t-0); spectra B-G
were taken at 1, 3, 7, 9, 27, and 54 h, respectively [66]
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations
carbohydrate moiety was assigned using the nomenclature introduced by Domon
and Costello, as A, B, C, X, Y, and Z [75]. An example of the mass spectrometry
characterization of neoglycoconjugate vaccines is discussed in the next section.
Fig. 11.5 The progress of conjugation of the hexasaccharide of Vibrio cholerae serotype Ogawa
(exact mass, 1780.79 Da) and BSA (molecular mass 66,430 Da) as revealed by monitoring the
reaction by SELDI-TOF MS. Spectrum A was taken at the onset of the reaction (t-0); spectra B-G
were taken at 1, 3, 7, 9, 27, and 54 h, respectively [66]
11 Defense Against Biological Terrorism: Vaccines and Their Characterizations