109
© Springer Nature B.V. 2020
G. Sindona et al. (eds.), Toxic Chemical and Biological Agents, NATO Science
for Peace and Security Series A: Chemistry and Biology,
https://doi.org/10.1007/978-94-024-2041-8_7
Chapter 7
Matrix-Assisted Laser Desorption/
Ionization Imaging Mass Spectrometry:
Technology and Applications
Josiah C. McMillen, William J. Perry, Kavya Sharman,
Katerina V. Djambazova, and Richard M. Caprioli
7.1 Introduction
Biological imaging modalities are commonly used for characterization of health
and disease, but each have trade-offs in the areas of spatial resolution, molecular
coverage and specificity, and targeted or untargeted approaches. Stained tissue
microscopy allows high spatial resolution to visualize tissue morphologies and cellular structure but provides little molecular information. Immunohistochemistry
allows high spatial resolution similar to that of organic stains with the benefit of
specific molecular information with the use of antibodies. However, molecular targets must be known in advance and antibodies may select for multiple molecular
species. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for the untargeted detection of hundreds to thousands of
J. C. McMillen · W. J. Perry · K. V. Djambazova
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, Vanderbilt University, Nashville, TN, USA
e-mail: josiah.c.mcmillen@vanderbilt.edu; william.j.perry@vanderbilt.edu;
katerina.v.djambazova@vanderbilt.edu
K. Sharman
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Program in Chemical and Physical Biology, Vanderbilt University, Nashville, TN, USA
e-mail: kavya.sharman@vanderbilt.edu
R. M. Caprioli (*)
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, Vanderbilt University, Nashville, TN, USA
Department of Biochemistry, Vanderbilt University, Nashville, TN, USA
Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, TN, USA
e-mail: r.caprioli@vanderbilt.edu
© Springer Nature B.V. 2020
G. Sindona et al. (eds.), Toxic Chemical and Biological Agents, NATO Science
for Peace and Security Series A: Chemistry and Biology,
https://doi.org/10.1007/978-94-024-2041-8_7
Chapter 7
Matrix-Assisted Laser Desorption/
Ionization Imaging Mass Spectrometry:
Technology and Applications
Josiah C. McMillen, William J. Perry, Kavya Sharman,
Katerina V. Djambazova, and Richard M. Caprioli
7.1 Introduction
Biological imaging modalities are commonly used for characterization of health
and disease, but each have trade-offs in the areas of spatial resolution, molecular
coverage and specificity, and targeted or untargeted approaches. Stained tissue
microscopy allows high spatial resolution to visualize tissue morphologies and cellular structure but provides little molecular information. Immunohistochemistry
allows high spatial resolution similar to that of organic stains with the benefit of
specific molecular information with the use of antibodies. However, molecular targets must be known in advance and antibodies may select for multiple molecular
species. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for the untargeted detection of hundreds to thousands of
J. C. McMillen · W. J. Perry · K. V. Djambazova
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, Vanderbilt University, Nashville, TN, USA
e-mail: josiah.c.mcmillen@vanderbilt.edu; william.j.perry@vanderbilt.edu;
katerina.v.djambazova@vanderbilt.edu
K. Sharman
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Program in Chemical and Physical Biology, Vanderbilt University, Nashville, TN, USA
e-mail: kavya.sharman@vanderbilt.edu
R. M. Caprioli (*)
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA
Department of Chemistry, Vanderbilt University, Nashville, TN, USA
Department of Biochemistry, Vanderbilt University, Nashville, TN, USA
Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, TN, USA
e-mail: r.caprioli@vanderbilt.edu